ANALYSIS OF ENLOPLATIN BY LIQUID-CHROMATOGRAPHY AND OF PLATINUM BY ATOMIC-ABSORPTION SPECTROMETRY IN VARIOUS BIOLOGICAL-FLUIDS

被引：6

作者：

AMORUSI, P ^{[1
]}

LESSARD, D ^{[1
]}

BANSAL, SK ^{[1
]}

SELINGER, K ^{[1
]}

YACOBI, A ^{[1
]}

TONELLI, AP ^{[1
]}

机构：

[1] PHOENIX INT LIFE SCI INC,MONTREAL H4R 2P7,PQ,CANADA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1994年 / 12卷 / 08期

关键词：

ENLOPLATIN; PLATINUM; REVERSED-PHASE LC; FLAMELESS ATOMIC ABSORPTION SPECTROMETRY (FAAS); ASSAY VALIDATION;

D O I：

10.1016/0731-7085(94)E0035-Y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Analytical methods were developed and validated for the determination of enloplatin (an anticancer agent) in plasma by reversed-phase LC and for platinum (an elemental component of enloplatin) in plasma, plasma ultrafiltrate (PUF) and whole blood by nameless atomic absorption spectrometry (FAAS). The LC procedure involved protein precipitation with dilute perchloric acid. The supernatant was mixed with sodium phosphate buffer and injected into the LC system. A C-18 or a cyano column was used, depending on sample matrix, with UV detection at 230 nm. The LC method was linear from 0.50 to 50.0 mu g ml(-1). Inter-day and intra-day precision (PSD%) and accuracy (relative error%) were <+/-14%. The FAAS procedure utilized a graphite furnace, a hollow cathode platinum (Pt) lamp, and Zeeman background correction. An aliquot of plasma, PUF, or whole blood was mixed with a solution of Triton X-100 and Antifoam-B and injected into the FAAS system. The FAAS method showed goodness of fit from 0.05 to 10.0 mu g Pt/ml. Inter-day and intra-day precision and accuracy were <+/-15%. The methods were developed to support pharmacokinetic studies in humans, dogs and rats.

引用

页码：1023 / 1033

页数：11

共 10 条

[1] RAPID-DETERMINATION OF PLATINUM BY FLAMELESS ATOMIC-ABSORPTION SPECTROPHOTOMETRY FOLLOWING THE ADMINISTRATION OF CISPLATIN TO CANCER-PATIENTS [J].