CONTRIBUTION OF THE CRYOPROTECTANT TO TOTAL INJURY IN RABBIT HEARTS FROZEN WITH ETHYLENE-GLYCOL

被引:20
作者
ARMITAGE, WJ
PEGG, DE
机构
[1] MRC Medical Cryobiology Group, University Department of Surgery, Cambridge, CB2 2AH England, Douglas House, Trumpington Rd
关键词
D O I
10.1016/0011-2240(79)90025-7
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Following the failure of hearts to recover function after freezing at -20 ° in the presence of 3 m ethylene glycol, a variety of experimental treatments was devised to determine the relative harmfulness of ice, high concentrations of electrolytes and high ethylene glycol concentration. Neither cooling to -20 °C without freezing in a Ca2+-free solution containing twice the normal salt concentration and 6 m ethylene glycol (freezing 3 m ethylene glycol at -20 °C doubles the solute concentration in the liquid phase), nor perfusion at -1 °C with this solution were conducive to the recovery of hearts. However, perfusion with Ca2+-free 3 m ethylene glycol solution with twice the normal concentration of salts did allow full recovery of function, whereas perfusion with Ca2+-free 6 m ethylene glycol solution with normal salt concentrations did not. Therefore, the high ethylene glycol concentration encountered during freezing was the main cause of damage. © 1979.
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页码:152 / 160
页数:9
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