STIMULATION OF PROLIFERATION AND IMMUNOGLOBULIN-M PRODUCTION BY LACTOFERRIN IN HUMAN-HUMAN AND MOUSE-MOUSE HYBRIDOMAS CULTURES IN SERUM-FREE CONDITIONS

被引：16

作者：

YAMADA, K

IKEDA, I

SUGAHARA, T

HASHIZUME, S

SHIRAHATA, S

MURAKAMI, H

机构：

[1] Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Fukuoka, 812, 6-10-1 Hakozaki, Higashi-ku

[2] Morinaga Institute of Biological Science, Yokohama, 230, Shimosueyoshi 2-1-1, Tsurumi-ku

[3] Graduate School of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, 812, 6-10-1 Hakozaki, Higashi-ku

来源：

CYTOTECHNOLOGY | 1990年 / 3卷 / 02期

关键词：

human-human hybridomas; lactoferrin; serum-free culture; stimulation of IgM production;

D O I：

10.1007/BF00143674

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of growth factors, such as insulin, transferrin, lactoferrin, ethanolamine, and selenium, on proliferation and IgM production of human-human hybridomas HB4C5 cells in a serum-free enriched RDF (eRDF) medium were studied. Among them, lactoferrin markedly stimulated proliferation and IgM production of the cells. Another iron-binding protein, transferrin, stimulated proliferation of HB4C5 cells as well as lactoferrin, but its stimulatory effect on IgM production was negligible. The proliferation and IgM production of HB4C5 cells gave some detectable delays in conventional ITES-eRDF medium at low cell densities, but the delays were avoided by the addition of lactoferrin. However, eRDF medium supplemented with lactoferrin could not support proliferation and IgM production of the cells at high cell densities. For proliferation and IgM production of HB4C5 cells, eRDF medium supplemented with 25 μg/ml lactoferrin, 10μM ethanolamine, 35 μ/ml transferrin, and 2.5 nM selenium (LETS-eRDF) gave the best result. Lactoferrin stimulated proliferation of human-human and mouse-mouse hybridomas producing IgG or IgM, but stimulation of Ig production was detected only in IgM producers. These results suggest that cell proliferation, IgM production, and IgG production of hybridomas are regulated by different mechanisms. © 1990 Kluwer Academic Publishers.

引用

页码：123 / 131

页数：9

共 28 条

[1]

Aihara K., Yamada K., Murakami H., Nomura Y., Omura H., Production of human-human hybridomas secreting monoclonal antibodies reactive to breast cancer cell lines, In Vitro Cell. Develop. Biol., 24, pp. 959-962, (1988)

[2]

Barnes D., Sato G., Serum-free cell culture: a unifying approach, Cell., 22, pp. 649-655, (1980)

[3]

Blazar B.A., Sutton L.M., Strome M., Self-stimulating growth factor production by B-cell lines derived from Burkitt's lymphomas and other lines transformed in vitro by Epstein-Barr virus, Cancer Res., 43, pp. 4562-4568, (1983)

[4]

Colcher D., Hand P.H., Nuti M., Schlom J., A spectram of monoclonal antibodies reactive with human mammary tumour cells, Proc. Natl. Acad. Sci. USA, 78, pp. 3199-3203, (1981)

[5]

Cuttitta F., Rosen S., Gazdar A.F., Monoclonal antibodies that demonstrate specificity for several types of human lung cancer, Proc. Natl. Acad. Sci. USA, 78, pp. 4591-4595, (1981)

[6]

Engvall E., Perlmann P., Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G, Immunochemistry, 8, pp. 871-874, (1971)

[7]

Gordon J., Ley S.C., Melamed M.D., English L.S., Hughes-Jones N.C., Immortalized B-lymphocytes produce B-cell growth factor, Nature, 310, pp. 145-147, (1984)

[8]

Harriman G.R., Kunimoto D.Y., Elliott J.F., Paetkau V., Strober W., The role of IL-5 in IgA B cell differentiation, J. Immunol., 140, pp. 3033-3039, (1988)

[9]

Hashizume S., Kuroda K., Murakami H., Identification of lactoferrin as an essential growth factor for lymphocytic cell lines in serum-free medium, Biochim. Biophys. Acta, 763, pp. 377-382, (1983)

[10]

Hashizume S., Murakami H., Hirose S., Shirai T., Shinohara K., Omura H., Human-human hybridomas from lung cancer patients secrete anti-DNA antibodies, Agricultural and Biological Chemistry, 50, pp. 1351-1353, (1986)

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