BIOCHEMICALLY ACTIVE SOL-GEL GLASSES - THE TRAPPING OF ENZYMES

被引：526

作者：

BRAUN, S ^{[1
]}

RAPPOPORT, S ^{[1
]}

ZUSMAN, R ^{[1
]}

AVNIR, D ^{[1
]}

OTTOLENGHI, M ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,INST CHEM,IL-91904 JERUSALEM,ISRAEL

来源：

MATERIALS LETTERS | 1990年 / 10卷 / 1-2期

关键词：

D O I：

10.1016/0167-577X(90)90002-4

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We describe the preparation and properties of a biochemically active sol-gel glass, obtained by trapping the enzyme alkaline phosphatase (ALP) in a polymerizing tetramethoxysilane. The immobilized purified ALP from bovine intestinal mucosa had a 30% activity yield and an improved stability to thermal deactivation compared to a solution. The composite bioactive glass was preserved in water at room temperature for two months without loosing activity. A non-Michaelis-Menten kinetics was observed. The concept of preparing bioactive materials by the sol-gel method seems to be general. Thus, other enzymes (chitinase, aspartase, β-glucosidase) were successfully trapped. © 1990.

引用

页码：1 / 5

页数：5

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