PHOSPHOLIPID MEMBRANE-PERMEABILITY OF PEPTIDE NUCLEIC-ACID

被引:120
作者
WITTUNG, P
KAJANUS, J
EDWARDS, K
NIELSEN, P
NORDEN, B
MALMSTROM, BG
机构
[1] UNIV UPPSALA,DEPT PHYS CHEM,S-75121 UPPSALA,SWEDEN
[2] UNIV COPENHAGEN,PANUM INST,CTR BIOMOLEC RECOGNIT,DEPT BIOCHEM B,DK-2200 COPENHAGEN,DENMARK
[3] GOTHENBURG UNIV,DEPT BIOCHEM & BIOPHYS,S-41390 GOTHENBURG,SWEDEN
关键词
LIPOSOME; PEPTIDE NUCLEIC ACID; CRYOGENIC TRANSMISSION ELECTRON MICROSCOPY; FLUORESCENCE;
D O I
10.1016/0014-5793(95)00409-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phospholipid vesicles (liposomes) as membrane models have been used to study the penetration properties of peptide nucleic acid (PNA), a new DNA analog in which the nucleobases are attached to a pseudo-peptide backbone, The liposomes were characterised by carboxyfluorescein efflux, light-scattering and cryogenic transmission electron microscopy. The liposome structure was found not to be affected by the incorporation of PNA or an oligonucleotide. Two 10-mer fluorescein-labelled PNAs were found to have low efflux rates (half-times of 5.5 and 11 days), comparable to a 10-mer oligonucleotide (half-time of 7 days). We conclude that passive diffusion of unmodified PNA is not an effective way of transport into biological cells.
引用
收藏
页码:27 / 29
页数:3
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