LANGERHANS CELL-DENSITY IN NORMAL HUMAN ORAL-MUCOSA AND SKIN - RELATIONSHIP TO AGE, SMOKING AND ALCOHOL-CONSUMPTION

The immune function of skin has been studied extensively and it has been suggested that epidermal Langerhans cell (LC) density and function decreases with increasing age. Little is known, however, about the effect of age on oral mucosal LC. Cryostat sections from biopsies of buccal mucosa, lip, hard palate, lateral border of tongue, floor of mouth and abdominal skin, obtained from 91 subjects (aged 16-96 yr), were reacted immunocytochemically with a monoclonal antibody against CD1a and then LC density was expressed as LC/mm epithelial surface length. No significant effect of age on mucosal or skin LC density was found, whilst a history of smoking was associated with an increase in LC density in lateral border of tongue and in biopsies of labial mucosa taken from men (P<0.05). There was no significant difference between LC density in men and women in oral mucosa. Oral mucosal LC may therefore form a relatively stable population in the adult and thus the increased incidence of mucosal disease in the elderly may be the result of subtle changes in cell mediated immune function rather than changes in LC density.