MICROCHIP CAPILLARY ELECTROPHORESIS WITH AN INTEGRATED POSTCOLUMN REACTOR

被引：371

作者：

JACOBSON, SC ^{[1
]}

KOUTNY, LB ^{[1
]}

HERGENRODER, R ^{[1
]}

MOORE, AW ^{[1
]}

RAMSEY, JM ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,DIV CHEM & ANALYT SCI,OAK RIDGE,TN 37831

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 20期

关键词：

D O I：

10.1021/ac00092a027

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A glass microchip with a postcolumn reactor was fabricated to conduct postseparation derivatization using o-phthaldialdehyde as a fluorescent ''tag'' for amino acids. This miniaturized separation device was constructed using standard photolithographic, wet chemical etching, and bonding techniques. Effects of the reagent stream on separation efficiency were investigated. In addition, a novel gated injector was demonstrated which maintains the integrity of the analyte, buffer, and reagent streams.

引用

页码：3472 / 3476

页数：5

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