NOTE ON THE ENUMERATION OF EPIPHYTIC BACTERIA BY MICROSCOPIC METHODS WITH PARTICULAR REFERENCE TO 2 FRESHWATER PLANTS

被引：40

作者：

HOSSELL, JC

BAKER, JH

机构：

[1] River Laboratory, Freshwater Biological Association, Wareham, Dorset, BH20 6BB, East Stoke

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1979年 / 46卷 / 01期

关键词：

D O I：

10.1111/j.1365-2672.1979.tb02584.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Bacteria on plant surfaces were examined using epifluorescence, bright‐field microscopy and an impression technique. Staining bacteria directly on the plant surface with phenolic aniline blue was found to be the best method to use for the determination of bacterial density. The effect on the estimation of population density of pretreatment of the plant with agents such as methanol and eosin yellowish was investigated. The average sizes of the bacterial populations on two freshwater plants, Rorippa and Lemna, estimated after staining by this method, were 5 times 106 and 9 times 106 bacteria cm‐z respectively. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：87 / 92

页数：6

共 18 条

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