RAPID MICROMETHOD FOR DETERMINATION OF N-15 ENRICHMENT IN PLASMA LYSINE - APPLICATION TO MEASUREMENT OF WHOLE-BODY PROTEIN-TURNOVER

被引：23

作者：

BIER, DM

CHRISTOPHERSON, HL

机构：

[1] Washington University School of Medicine, Metabolism Division, St. Louis, MO 63110

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90354-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, sensitive method, requiring less than 1 ml of blood, is deseribed for measurement of stable isotope enrichment in plasma lysine with a relative precision of better than ±0.5% of the observed value. A measurement of whole body protein turnover obtained by this method was virtually identical to values obtained by more tedious macromethods. The speed of the reported procedure allows its application to routine clinical studies which generate a large number of samples. © 1979.

引用

页码：242 / 248

页数：7

共 39 条

[1]

BENEDETTI MS, 1975, 2ND P INT C STABL IS, P511

[2] TRIMETHYLSILYLATION OF AMINO ACIDS .1. STUDY OF GLYCINE AND LYSINE TMS DERIVATIVES WITH GAS-LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY [J].