TISSUE-SPECIFIC EXPRESSION OF TYPE-XII COLLAGEN DURING MOUSE EMBRYONIC-DEVELOPMENT

被引：69

作者：

OH, SP ^{[1
]}

GRIFFITH, CM ^{[1
]}

HAY, ED ^{[1
]}

OLSEN, BR ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT ANAT & CELLULAR BIOL,220 LONGWOOD AVE,BOSTON,MA 02115

来源：

DEVELOPMENTAL DYNAMICS | 1993年 / 196卷 / 01期

关键词：

D O I：

10.1002/aja.1001960105

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Polyclonal antibodies were raised in rabbits against a fusion peptide representing a portion of the amino-terminal non-triple-helical domain of mouse type XII collagen. The antibodies reacted with bands of 220 and 350 kDa on Western blots of mouse tissue extracts. Immunohistochemical analyses of mouse embryos demonstrated that type XII collagen is expressed mainly in dense connective tissues of tendons, ligaments, dermis, cornea, blood vessel walls, meninges, and developing membranous bones. Comparison of skin extracts and medium of cultured mouse skin fibroblasts by Western blotting showed that while tissues contain short 220 kDa type XII collagen polypeptides as well as the long form, cultured cells produce mainly the long form with 350 kDa polypeptides.

引用

页码：37 / 46

页数：10

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