EFFECT OF CHRONIC ETHANOL ON CALCIUM CURRENTS AND CALCIUM-UPTAKE IN UNDIFFERENTIATED PC12 CELLS

被引：41

作者：

GRANT, AJ

KOSKI, G

TREISTMAN, SN

机构：

[1] UNIV MASSACHUSETTS, MED CTR, DEPT PHARMACOL, 55 LAKE AVE N, WORCESTER, MA 01655 USA

[2] WORCESTER FDN EXPTL BIOL INC, SHREWSBURY, MA 01545 USA

[3] MASSACHUSETTS GEN HOSP, DEPT ANESTHESIA, HENRY K BEECHER MEM RES LABS, BOSTON, MA 02114 USA

来源：

BRAIN RESEARCH | 1993年 / 600卷 / 02期

关键词：

PHEOCHROMOCYTOMA; PC12; ETHANOL; CALCIUM FLUX; PATCH CLAMP; TOLERANCE;

D O I：

10.1016/0006-8993(93)91384-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Undifferentiated pheochromocytoma (PC12) cells were chronically exposed to 200 mM ethanol for six days. Parallel cultures were maintained without ethanol. Cells chronically exposed to ethanol had significantly larger voltage-gated calcium currents than those grown in the absence of ethanol. Concurrent radioisotopic flux assays confirm that calcium influx is, indeed, increased in the chronically treated cells. However, acute exposure to ethanol at the same concentration as those used for the chronic studies greatly reduced the magnitude of the currents and net calcium influx.

引用

页码：280 / 284

页数：5

共 31 条

[1] ETHANOL-INDUCED REDUCTION OF NEUROANAL CALCIUM CURRENTS IN APLYSIA - AN EXAMINATION OF POSSIBLE MECHANISMS [J].