DNA EXTRACTION FOR 16S RIBOSOMAL-RNA GENE ANALYSIS TO DETERMINE GENETIC DIVERSITY IN DEEP SEDIMENT COMMUNITIES

被引：69

作者：

ROCHELLE, PA ^{[1
]}

FRY, JC ^{[1
]}

PARKES, RJ ^{[1
]}

WEIGHTMAN, AJ ^{[1
]}

机构：

[1] UNIV BRISTOL, DEPT GEOL, BRISTOL BS8 1TH, AVON, ENGLAND

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 100卷 / 1-3期

基金：

英国自然环境研究理事会;

关键词：

DNA EXTRACTION; POLYMERASE CHAIN REACTION; 16S RIBOSOMAL-RNA GENE; MARINE SEDIMENT;

D O I：

10.1016/0378-1097(92)90188-T

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A protocol was devised which permitted the extraction of DNA from deep marine sediments up to 503 m below the sea floor. These sediments have been laid down over the last 3 million years. 16S rRNA gene sequences were amplified from the DNA by the polymerase chain reaction. The details of the successful extraction and polymerase chain reaction methodology varied between samples from different depths. This emphasizes the attention to detail required to allow the diversity of bacteria in these deep sediments to be studied.

引用

页码：59 / 65

页数：7

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