BIOANALYSIS OF DIGOXIN AND ITS METABOLITES USING DIRECT SERUM INJECTION COMBINED WITH LIQUID-CHROMATOGRAPHY AND ONLINE IMMUNOCHEMICAL DETECTION

被引：52

作者：

OOSTERKAMP, AJ

IRTH, H

BETH, M

UNGER, KK

TJADEN, UR

VANDEGREEF, J

机构：

[1] LEIDEN UNIV,DIV ANALYT CHEM,LEIDEN AMSTERDAM CTR DRUG RES,POB 9502,2300 RA LEIDEN,NETHERLANDS

[2] UNIV MAINZ,INST ANORGAN & ANALYT CHEM,D-55099 MAINZ,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 653卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)E0405-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An automated dual-column liquid chromatographic assay for digoxin is described. Serum samples are directly injected onto a restricted-access solid-phase extraction support. After liquid chromatographic (LC) separation on a C18 analytical column, antigenic analytes are detected by means of post-column immunochemical detection (ICD) using fluorescein-labelled antibodies against digoxigenin. The detection limit of this assay is 160 pg/ml (preconcentration of 1.0 ml serum). With the present method digoxin and three of its cross-reactive metabolites were determined in serum taken from patients which were orally administered a 1-mg dose of digoxin. The results obtained with LC-ICD were compared with data provided by a batch immunoassay.

引用

页码：55 / 61

页数：7

共 13 条

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