CONSTITUTIVE EXPRESSION OF HEAT-SHOCK PROTEIN-90 (HSP90) IN NEURONS OF THE RAT-BRAIN

被引：31

作者：

GASS, P ^{[1
]}

SCHRODER, H ^{[1
]}

PRIOR, P ^{[1
]}

KIESSLING, M ^{[1
]}

机构：

[1] UNIV COLOGNE,DEPT ANAT,COLOGNE,GERMANY

来源：

NEUROSCIENCE LETTERS | 1994年 / 182卷 / 02期

关键词：

HSP90; IMMUNOCYTOCHEMISTRY; IMMUNOBLOTTING; IMMUNE-ELECTRON MICROSCOPY;

D O I：

10.1016/0304-3940(94)90794-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Immunoblot analysis, immunocytochemistry and immune-electron microscopy were employed to study the expression of HSP90 protein in the adult rat brain, using a specific polyclonal antiserum. Immunoblot analysis demonstrated equal levels of HSP90 in microdissected extracts from hippocampus, cortex, striatum and cerebellum. Immunocytochemistry and immune-electron microscopy provided evidence that HSP90 is markedly expressed thoughout all neuronal subpopulations of the CNS but not in non-neuronal cells except ependyma and choroid plexus. At the ultrastructural level, HSP90 immunoreactivity was predominantly found in perikarya but to a lesser extent also in dendrites and nuclei. The constitutive expression of HSP90 in widespread neuronal cell populations suggests a functional role in the physiological molecular program of CNS neurons.

引用

页码：188 / 192

页数：5

共 20 条

[1] HYPERTHERMIA PROTECTS AGAINST LIGHT DAMAGE IN THE RAT RETINA [J].