ONLINE MONITORING OF MONOCLONAL-ANTIBODY PRODUCTION WITH REGENERABLE FLOW-INJECTION IMMUNO-SYSTEMS

被引：21

作者：

GEBBERT, A ^{[1
]}

ALVAREZICAZA, M ^{[1
]}

PETERS, H ^{[1
]}

JAGER, V ^{[1
]}

BILITEWSKI, U ^{[1
]}

SCHMID, RD ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,DEPT ENZYME TECHNOL,MASCHERODER WEG 1,D-38124 BRAUNSCHWEIG,GERMANY

来源：

JOURNAL OF BIOTECHNOLOGY | 1994年 / 32卷 / 03期

关键词：

FLOW-INJECTION IMMUNO-SYSTEM (FIIA); MONOCLONAL ANTIBODY; ONLINE MONITORING; CAPACITANCE SENSOR; FLUOROMETRIC SENSOR;

D O I：

10.1016/0168-1656(94)90207-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this paper two systems for the observation of the production of mouse-IgG during the cultivation of hybridoma cells in a perfusion reactor are presented. The direct immunosystem is based on the detection of changes in capacitance of a dielectric layer (tantalum oxide) on a metal surface (tantalum) when antibodies bind to immobilized anti-antibodies. The sensor consisted of a 25 nm tantalum oxide layer, electrochemically grown onto a laser patternized 1 mum thick tantalum layer. The indirect system is based on an automated fluorimetric sandwich ELISA system with beta-galactosidase conjugated secondary antibodies. Two cultivations of mouse hybridoma cells in a 2-1 perfusion reactor were performed. The first cultivation was monitored with the capacitance system, the second cultivation was monitored with the fluorimetric system.

引用

页码：213 / 220

页数：8

共 28 条

[1] A CHEMICALLY STERILIZABLE BARRIER FOR THE PROTECTION OF FERMENTERS DURING AUTOMATIC SAMPLING INTO A FLOW-INJECTION SYSTEM [J].

APPELQVIST, R ;

JOHANSSON, G ;

HOLST, O ;

MATTIASSON, B .

ANALYTICA CHIMICA ACTA, 1989, 216 (1-2) :299-306

[2] TOXIN DETECTION USING CAPACITANCE MEASUREMENTS ON IMMUNOSPECIES GRAFTED ONTO A SEMICONDUCTOR SUBSTRATE [J].

BILLARD, V ;

MARTELET, C ;

BINDER, P ;

THERASSE, J .

ANALYTICA CHIMICA ACTA, 1991, 249 (02) :367-372

[3]

BLASEY HD, 1991, ANIMAL CELL CULTURE AND PRODUCTION OF BIOLOGICALS, P61

[4] USE OF MONOCLONAL-ANTIBODIES TO T-CELL SUBSETS FOR IMMUNOLOGICAL MONITORING AND TREATMENT IN RECIPIENTS OF RENAL-ALLOGRAFTS [J].

COSIMI, AB ;

COLVIN, RB ;

BURTON, RC ;

RUBIN, RH ;

GOLDSTEIN, G ;

KUNG, PC ;

HANSEN, WP ;

DELMONICO, FL ;

RUSSELL, PS .

NEW ENGLAND JOURNAL OF MEDICINE, 1981, 305 (06) :308-314

[5] IMMUNO AND FLOW CYTOMETRIC ANALYTICAL METHODS FOR BIOTECHNOLOGICAL RESEARCH AND PROCESS MONITORING [J].

DEGELAU, A ;

FREITAG, R ;

LINZ, F ;

MIDDENDORF, C ;

SCHEPER, T ;

BLEY, T ;

MULLER, S ;

STOLL, P ;

REARDON, KF .

JOURNAL OF BIOTECHNOLOGY, 1992, 25 (1-2) :115-144

[6]

FENGE C, 1993, IN PRESS CYTOTECHNOL

[7]

FREITAG R, 1991, APPL MICROBIOL BIOT, V35, P471, DOI 10.1007/BF00169752

[8] IMMUNOLOGICAL ONLINE DETECTION OF SPECIFIC PROTEINS DURING FERMENTATION PROCESSES [J].

FREITAG, R ;

FENGE, C ;

SCHEPER, T ;

SCHUGERL, K ;

SPREINAT, A ;

ANTRANIKIAN, G ;

FRAUNE, E .

ANALYTICA CHIMICA ACTA, 1991, 249 (01) :113-122

[9] REAL-TIME MONITORING OF IMMUNOCHEMICAL INTERACTIONS WITH A TANTALUM CAPACITANCE FLOW-THROUGH CELL [J].

GEBBERT, A ;

ALVAREZICAZA, M ;

STOCKLEIN, W ;

SCHMID, RD .

ANALYTICAL CHEMISTRY, 1992, 64 (09) :997-1003

[10]

GEBBERT A, 1992, DECHEMA BIOTECHNOL, V5, P489

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