STATIC CHARACTERISTICS OF A CONTINUOUS-FLOW BIOREACTOR CONTAINING ANTIBIOTIC-RESISTANT RECOMBINANT CELLS

被引：9

作者：

CHANG, YK

LIM, HC

机构：

[1] Purdue Univ, West Lafayette, IN, USA, Purdue Univ, West Lafayette, IN, USA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1987年 / 29卷 / 08期

关键词：

BIOREACTORS - Mathematical Models - CELL CULTURE - Growth Kinetics - DRUG PRODUCTS - Antibiotics - Genetic engineering - Metabolism;

D O I：

10.1002/bit.260290806

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Steady state behavior of continuous recombinant cultures fed with a medium containing an antibiotic is examined in the present article. The stability characteristics, including possible occurence of static bifurcation and Hopf bifurcation of the possible steady states are investigated. Sensitivity analyses for several kinetic parameters, such as susceptibility of the plasmid-free cells to antibiotic, are carried out. An optimization to find the optimal antibiotic feed concentration for maximum profit are described briefly for a specific set of system parameters.

引用

页码：950 / 961

页数：12

共 34 条

[1]

ADAMS J, 1979, GENETICS, V91, P627

[2] EFFECTS OF TEMPERATURE ON PLASMID STABILITY AND PENICILLINASE PRODUCTIVITY IN A TRANSFORMANT OF BACILLUS-STEAROTHERMOPHILUS [J].