MODULAR DESIGN OF SYNTHETIC PROTEIN MIMICS - CRYSTAL-STRUCTURE OF 2 7-RESIDUE HELICAL PEPTIDE SEGMENTS LINKED BY EPSILON-AMINOCAPROIC ACID

被引:72
作者
KARLE, IL [1 ]
FLIPPENANDERSON, JL [1 ]
SUKUMAR, M [1 ]
UMA, K [1 ]
BALARAM, P [1 ]
机构
[1] INDIAN INST SCI, MOLEC BIOPHYS UNIT, BANGALORE 560012, KARNATAKA, INDIA
关键词
D O I
10.1021/ja00010a042
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Two seven-residue helical segments, Val-Ala-Leu-Aib-Val-Ala-Leu, were linked synthetically with an epsilon-aminocaproic acid (Acp) linker with the intention of making a stable antiparallel helix-helix motif. The crystal structure of the linked peptide Boc-Val-Ala-Leu-Aib-Val-Ala-Leu-Acp-Val-Ala-Leu-Aib-Val-Ala-Leu-OMe (1) shows the two helices displaced laterally from each other by the linker, but the linker has not folded the molecule into a close-packed antiparallel conformation. Two strong intermolecular NH...O = C hydrogen bonds are formed between the top of the lower helix of one molecule and the bottom of the upper helix in a laterally adjacent molecule to give the appearance of an extended single helix. The composite peptide with Boc and OMe end groups, C76H137N15O18.H2O, crystallize in space group P2(1) with a = 8.802 (1) angstrom, b = 20.409 (4) angstrom, c = 26.315 (3) angstrom, and beta = 90.72 (1)degrees; overall agreement R = 7.86% for 5030 observed reflections (\F(o)\ > 3-sigma(F)); resolution = 0.93 angstrom. Limited evidence for a more compact conformation in solution consistent with an antiparallel helix arrangement is obtained by comparison of the HPLC retention times and CD spectra of peptide 1 with well-characterized continuous helices of similar length and sequence.
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页码:3952 / 3956
页数:5
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