DIFFERENT TBP-ASSOCIATED FACTORS ARE REQUIRED FOR MEDIATING THE STIMULATION OF TRANSCRIPTION INVITRO BY THE ACIDIC TRANSACTIVATOR GAL-VP16 AND THE 2 NONACIDIC ACTIVATION FUNCTIONS OF THE ESTROGEN-RECEPTOR

被引：69

作者：

BROU, C ^{[1
]}

WU, J ^{[1
]}

ALI, S ^{[1
]}

SCHEER, E ^{[1
]}

LANG, C ^{[1
]}

DAVIDSON, I ^{[1
]}

CHAMBON, P ^{[1
]}

TORA, L ^{[1
]}

机构：

[1] FAC MED STRASBOURG,INST CHIM BIOL,GENET MOLEC EUCARYOTES LAB,CNRS,UA 184,INSERM,11 RUE HUMANN,F-67085 STRASBOURG,FRANCE

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 01期

关键词：

D O I：

10.1093/nar/21.1.5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The estrogen receptor (ER) contains two nonacidic transcriptional activation functions, AF-1 and AF-2 (formerly TAF-1 and TAF-2). In this study we show that AF-1 and AF-2 are able to stimulate transcription in vitro in a HeLa cell system when fused to the DNA binding domain of the yeast activator GAL4. We also demonstrate that a factor(s) required for the function of the ER AFs is chromatographically separable from a factor(s) necessary for the activity of the acidic activation domain of VP16. Moreover, immunoprecipitation experiments using a monoclonal antibody directed against the TATA box binding protein (TBP) indicate, that these different factors are associated with TBP in distinct TFIID complexes.

引用

页码：5 / 12

页数：8

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