PROLIFERATION OF HUMAN LEUKEMIC PRE-B CELLS INDUCED BY HUMAN BONE-MARROW STROMAL CELLS AND MURINE FIBROBLASTS

被引：7

作者：

BUSKE, C ^{[1
]}

HIDDEMANN, W ^{[1
]}

WORMANN, B ^{[1
]}

机构：

[1] UNIV GOTTINGEN,DEPT INTERNAL MED,DIV HEMATOL ONCOL,D-37075 GOTTINGEN,GERMANY

来源：

CELL BIOLOGY INTERNATIONAL | 1994年 / 18卷 / 11期

关键词：

D O I：

10.1006/cbir.1994.1028

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Aim of the study was the establishment of a standardized in vitro culture system for studies on proliferation and differentiation of human leukemic pre - B cells. Coincubation with human stromal cells led to a significantly higher proliferation of the cytokine - sensitive leukemic pre - B cell line BLIN-1. Clones from the murine fibroblast cell line L929 provided identical results. Coincubation with the murine cells also resulted in a significantly higher numbers of viable cells in 5 of 8 patient samples with newly diagnosed B lineage ALL. The results show that in vitro bone marrow stromal cells can be substituted by murine fibroblasts and form the basis for a simpler and more reproducible assay system.

引用

页码：1049 / 1058

页数：10

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