PREPARATION OF I-125-LABELED BILE-ACID LIGANDS FOR USE IN THE RADIOIMMUNOASSAY OF BILE-ACIDS

被引：57

作者：

BECKETT, GJ

CORRIE, JET

PERCYROBB, IW

机构：

[1] UNIV EDINBURGH, ROYAL INFIRM, DEPT CLIN CHEM, EDINBURGH EH3 9YW, MIDLOTHIAN, SCOTLAND

[2] MRC, RADIOIMMUNOASSAY TEAM, EDINBURGH EH1 2QW, MIDLOTHIAN, SCOTLAND

来源：

CLINICA CHIMICA ACTA | 1979年 / 93卷 / 01期

关键词：

D O I：

10.1016/0009-8981(79)90255-9

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A general method for the preparation of 125I-labeled bile acid-histamine or 125I-labeled bile acid-tyramine conjugates is presented. The method is simple, quick and produces ligands in good yield (30%). The characteristics of a radioimmunoassay for conjugated chenodeoxycholic acid, based on an 125I-labeled ligand prepared by the method, are also described. The assay produces values for fasting [human] serum concentrations of conjugated chenodeoxycholic acid that agree well with previous data.

引用

页码：145 / 150

页数：6

共 8 条

[1] INVESTIGATIONS INTO CHOICE OF IMMUNOGEN, LIGAND, ANTISERUM AND ASSAY CONDITIONS FOR RADIOIMMUNOASSAY OF CONJUGATED CHOLIC-ACID
BECKETT, GJ
HUNTER, WM
PERCYROBB, IW
[J]. CLINICA CHIMICA ACTA, 1978, 88 (02) : 257 - 266
[2] DAWSON RMC, 1969, DATA BIOCHEMICAL RES, P530
[3] HUNTER WM, 1975, 5TH P TEN WORKSH, P141
[4] DYNAMICS OF ENTEROHEPATIC CIRCULATION OF BILE-ACIDS - POSTPRANDIAL SERUM CONCENTRATIONS OF CONJUGATES OF CHOLIC ACID IN HEALTH, CHOLECYSTECTOMIZED PATIENTS, AND PATIENTS WITH BILE-ACID MALABSORPTION
LARUSSO, NF
KORMAN, MG
HOFFMAN, NE
HOFMANN, AF
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1974, 291 (14) : 689 - 692
[5] PREPARATION AND PROPERTIES OF AN ANTISERUM FOR RADIOIMMUNOASSAY OF SERUM CONJUGATED CHOLIC ACID
MURPHY, GM
EDKINS, SM
WILLIAMS, JW
CATTY, D
[J]. CLINICA CHIMICA ACTA, 1974, 54 (01) : 81 - 89
[6] SCHALM SW, 1975, GASTROENTEROLOGY, V68, P913
[7] SIMMONDS WJ, 1973, GASTROENTEROLOGY, V65, P705
[8] SPENNEY JG, 1977, GASTROENTEROLOGY, V72, P305

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