DETERMINATION OF HYALURONIC ACID IN MICROGRAM RANGE

被引：12

作者：

LAURENT, TC

BARANY, E

CARLSSON, B

TIDARE, E

机构：

[1] Department of Medical Chemistry, University of Uppsala

[2] Department of Pharmacology, University of Uppsala

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 31卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90250-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An isotope dilution method has been developed for the determination of hyaluronic acid in tissue samples in the microgram range. The unknown sample is mixed with 14C-labeled hyaluronic acid, proteins are removed by proteolytic digestion and gel chromatography, and the hyaluronic acid is degraded to unsaturated disaccharides by a streptococcal hyaluronate lyase. The disaccharides are then tritiated catalytically and isolated by high-voltage paper electrophoresis. The ratio of 3H to 14C is a linear function of the amount of hyaluronic acid in the sample. The lower limit for the analysis is at present approximately 1 mμeq (0.4 μg) of repeating disaccharide units. The technique has been applied to the analysis of hyaluronic acid in the aqueous humor. © 1969.

引用

页码：133 / &

共 24 条

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