GENETIC MICRO-SURGERY BY LASER - ESTABLISHMENT OF A CLONAL POPULATION OF RAT KANGAROO CELLS (PTK2) WITH A DIRECTED DEFICIENCY IN A CHROMOSOMAL NUCLEOLAR ORGANIZER

被引：25

作者：

BERNS, MW

CHONG, LK

HAMMERWILSON, M

MILLER, K

SIEMENS, A

机构：

[1] Developmental and Cell Biology, University of California, Irvine, Irvine, 92717, California

来源：

CHROMOSOMA | 1979年 / 73卷 / 01期

关键词：

D O I：

10.1007/BF00294839

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An ultraviolet laser beam was focused to a submicron spot on one of the nucleolar organizer regions of mitotic chromosomes of rat kangaroo cells in tissue culture. The daughter cells were isolated and cloned into a viable population that maintained the directed nucleolar deficiency. It is concluded that the laser can be used to delete preselected genetic regions and the genetic deletion is maintained as a heritable deficiency in subsequent daughter cells. © 1979 Springer-Verlag.

引用

页码：1 / 8

页数：8

共 14 条

[1] Berns M W, 1978, Methods Cell Biol, V18, P277
[2] IN VITRO PRODUCTION OF CHROMOSOMAL LESIONS WITH AN ARGON LASER MICROBEAM
BERNS, MW
OLSON, RS
ROUNDS, DE
[J]. NATURE, 1969, 221 (5175) : 74 - &
[3] DIRECTED CHROMOSOME LOSS BY LASER MICROIRRADIATION
BERNS, MW
[J]. SCIENCE, 1974, 186 (4165) : 700 - 705
[4] MODIFICATION OF NUCLEOLAR EXPRESSION FOLLOWING LASER MICRO-IRRADIATION OF CHROMOSOMES
BERNS, MW
OHNUKI, Y
ROUNDS, DE
OLSON, RS
[J]. EXPERIMENTAL CELL RESEARCH, 1970, 60 (01) : 133 - &
[5] LASER MICROIRRADIATION OF NUCLEOLAR ORGANIZER IN CELLS OF RAT KANGAROO (POTOROUS-TRIDACTYLIS) - REDUCTION OF NUCLEOLAR NUMBER AND PRODUCTION OF MICRONUCLEOLI
BERNS, MW
FLOYD, AD
ADKISSON, K
CHENG, WK
MOORE, L
HOOVER, G
USTICK, K
BURGOTT, S
OSIAL, T
[J]. EXPERIMENTAL CELL RESEARCH, 1972, 75 (02) : 424 - 432
[6] ARE CHROMOSOME SECONDARY SONSTRICTIONS NUCLEOLAR ORGANIZERS - RE-EXAMINATION USING A LASER MICROBEAM
BERNS, MW
CHENG, WK
[J]. EXPERIMENTAL CELL RESEARCH, 1971, 69 (01) : 185 - &
[7] PARTIAL CELL IRRADIATION WITH A TUNABLE ORGANIC-DYE LASER
BERNS, MW
[J]. NATURE, 1972, 240 (5382) : 483 - &
[8] LASER MICROIRRADIATION OF CHROMOSOMES
BERNS, MW
[J]. COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1973, 38 : 165 - 174
[9] RECENT PROGRESS WITH LASER MICROBEAMS
BERNS, MW
[J]. INTERNATIONAL REVIEW OF CYTOLOGY-A SURVEY OF CELL BIOLOGY, 1974, 39 : 383 - 411
[10] BERNS MW, CELL BIOPHYSICS

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