IMMUNOGOLD LIGHT AND ELECTRON-MICROSCOPIC DETECTION OF AMYLOID PLAQUES IN TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES

被引：12

作者：

DOERRSCHOTT, J

KITAMOTO, T

TATEISHI, J

BOELLAARD, JW

HELDT, N

LICHTE, C

机构：

[1] KYUSHU UNIV,FAC MED,INST NEUROL,DEPT NEUROPATHOL,FUKUOKA 812,JAPAN

[2] UNIV TUBINGEN,INST HIRNFORSCH,W-7400 TUBINGEN 1,GERMANY

[3] UNIV STRASBOURG 1,HOP HAUTEPIERRE,SERV PATHOL,F-67070 STRASBOURG,FRANCE

来源：

NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY | 1990年 / 16卷 / 01期

关键词：

amyloid plaques; colloidal gold; Gerstmann‐Sträussler syndrome; immuno‐light and immuno‐electron microscopy; infected Creutzfeldt‐Jakob mouse; silver intensification; spongiform encephalopathies; prion’ protein;

D O I：

10.1111/j.1365-2990.1990.tb00934.x

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

The antigenicity of the ‘prion’ protein amyloid fibrils was shown to be preserved after glutaraldehyde/OsO4 fixation in uranyl acetate‐ stained brain tissue blocks from patients with Gerstmann‐Straussler syndrome (GSS) and from mice infected with Creutzfeldt‐Jakob disease (CJD). Amyloid plaques were demonstrated by light microscopy in immunogold silver‐intensified semithin sections. Under the electron microscope, the amyloid fibrils were labelled in immunogold‐reacted ultrathin sections using an antiserum prepared against GSS amyloid plaque cores and mouse amyloid fibrils respectively. The influence of various oxidizing agents (hydrogen peroxide, sodium metaperiodate) on the tissue preservation and the immunohistochemical detection was tested. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：85 / 89

页数：5

共 10 条

[1] INFLUENCE OF HYDROGEN-PEROXIDE OR ALCOHOLIC SODIUM-HYDROXIDE ON THE IMMUNOCYTOCHEMICAL DETECTION OF GROWTH-HORMONE AND PROLACTIN AFTER OSMIUM FIXATION [J].