C-TERMINAL KDEL-MODIFIED CYSTATIN-C IS RETAINED IN TRANSFECTED CHO CELLS

被引：14

作者：

JOHANSEN, TE

VOGEL, CK

SCHWARTZ, TW

机构：

[1] Laboratory of Molecular Endocrinology, University Department of Clinical Chemistry, Rigshospitalet 6321, DK-2100 Copenhagen

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)91603-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The significance of a C-terminal tetrapeptide, Lys-Asp-Glu-Leu (KDEL), as a retention signal for the endoplasmatic reticulum was studied using cystatin C, a general thiol protease inhibitor, as the reporter protein. Clones of CHO cells were analyzed after stable transfection with eukaryotic expression vectors encoding either cystatin C, KDEL extended cystatin C, or cystatin C extended with a control sequence. It is concluded that cystatin C with the KDEL tetrapeptide as a C-terminal extension is retained intracellularly without apparent accumulation of the molecule. © 1990.

引用

页码：1384 / 1391

页数：8

共 18 条

[1] EFFICIENT PRODUCTION OF NATIVE, BIOLOGICALLY-ACTIVE HUMAN CYSTATIN-C BY ESCHERICHIA-COLI
ABRAHAMSON, M
DALBOGE, H
OLAFSSON, I
CARLSEN, S
GRUBB, A
[J]. FEBS LETTERS, 1988, 236 (01) : 14 - 18
[2] MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF CDNA CODING FOR THE PRECURSOR OF THE HUMAN CYSTEINE PROTEINASE-INHIBITOR CYSTATIN-C
ABRAHAMSON, M
GRUBB, A
OLAFSSON, I
LUNDWALL, A
[J]. FEBS LETTERS, 1987, 216 (02) : 229 - 233
[3] ABRAHAMSON M, 1986, J BIOL CHEM, V261, P1282
[4] NEW TECHNIQUE FOR ASSAY OF INFECTIVITY OF HUMAN ADENOVIRUS 5 DNA
GRAHAM, FL
VANDEREB, AJ
[J]. VIROLOGY, 1973, 52 (02) : 456 - 467
[5] GRUB A, 1982, P NATL ACAD SCI USA, V791, P3024
[6] ERD1, A YEAST GENE REQUIRED FOR THE RETENTION OF LUMINAL ENDOPLASMIC-RETICULUM PROTEINS, AFFECTS GLYCOPROTEIN PROCESSING IN THE GOLGI-APPARATUS
HARDWICK, KG
LEWIS, MJ
SEMENZA, J
DEAN, N
PELHAM, HRB
[J]. EMBO JOURNAL, 1990, 9 (03) : 623 - 630
[7] BIOSYNTHESIS OF PEPTIDE PRECURSORS AND PROTEASE INHIBITORS USING NEW CONSTITUTIVE AND INDUCIBLE EUKARYOTIC EXPRESSION VECTORS
JOHANSEN, TE
SCHOLLER, MS
TOLSTOY, S
SCHWARTZ, TW
[J]. FEBS LETTERS, 1990, 267 (02) : 289 - 294
[8] CLONING, CHARACTERIZATION, AND DNA-SEQUENCE OF A HUMAN CDNA-ENCODING NEUROPEPTIDE TYROSINE
MINTH, CD
BLOOM, SR
POLAK, JM
DIXON, JE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (14): : 4577 - 4581
[9] A C-TERMINAL SIGNAL PREVENTS SECRETION OF LUMINAL ER PROTEINS
MUNRO, S
PELHAM, HRB
[J]. CELL, 1987, 48 (05) : 899 - 907
[10] CONTROL OF PROTEIN EXIT FROM THE ENDOPLASMIC-RETICULUM
PELHAM, HRB
[J]. ANNUAL REVIEW OF CELL BIOLOGY, 1989, 5 : 1 - 23

← 1 2 →