STRUCTURAL DYNAMICS IN AN ELECTRON-TRANSFER COMPLEX

被引：36

作者：

JENG, MF ^{[1
]}

ENGLANDER, SW ^{[1
]}

PARDUE, K ^{[1
]}

ROGALSKYJ, JS ^{[1
]}

MCLENDON, G ^{[1
]}

机构：

[1] UNIV ROCHESTER,DEPT CHEM,ROCHESTER,NY 14627

来源：

NATURE STRUCTURAL BIOLOGY | 1994年 / 1卷 / 04期

关键词：

D O I：

10.1038/nsb0494-234

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The dynamic behaviour of the complex of house cytochrome c with cytochrome c peroxidase, an electron-transfer complex, was studied in solution by a hydrogen exchange labelling method together with two-dimensional NMR analysis. Although cytochrome c hydrogens in the expected binding region exhibit slowed exchange, the measured slowing factors ave very small, indicating that hydrogen-exchange occurs with little hindrance from within the binding interface. The complex in solution must therefore be highly mobile rather than rigidly defined, as implied by the crystalline complex. This result is in conflict with the concept that biological electron transfer occurs by way of predetermined covalent pathways.

引用

页码：234 / 238

页数：5

共 43 条

[1] 2-DIMENSIONAL SPECTROSCOPY - APPLICATION TO NUCLEAR MAGNETIC-RESONANCE [J].