STIMULATION BY BONE SIALOPROTEIN OF CALCIFICATION IN OSTEOBLAST-LIKE MC3T3-E1 CELLS

被引：73

作者：

ZHOU, HY ^{[1
]}

TAKITA, H ^{[1
]}

FUJISAWA, R ^{[1
]}

MIZUNO, M ^{[1
]}

KUBOKI, Y ^{[1
]}

机构：

[1] HOKKAIDO UNIV,SCH DENT,DEPT BIOCHEM,SAPPORO,HOKKAIDO 060,JAPAN

来源：

CALCIFIED TISSUE INTERNATIONAL | 1995年 / 56卷 / 05期

关键词：

BONE SIALOPROTEIN; FIBRONECTIN; TYPE I COLLAGEN; MC3T3-E1; CELLS; CALCIFICATION;

D O I：

10.1007/BF00301610

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

Bone sialoprotein (BSP) containing an Arg-Gly-Asp cell-binding sequence was purified from bovine bone 4 M guanidine-HCl extract after HCl demineralization by a series of chromatographic procedures. When this protein was coated on culture dishes in the presence of type I collagen, it increased both DNA content and alkaline phosphatase (ALP) activity in osteoblast-like MC3T3-E1 cells, and stimulated calcification in the cells, whereas fibronectin, another cell-binding protein, showed a marked increase in the DNA content but had little effect on the ALP activity. These findings suggest that BSP is mitogenic for preosteoblasts and differentiating the cells into osteoblasts, thereby stimulating bone calcification.

引用

页码：403 / 407

页数：5

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