INTRACELLULAR PROTEINS OF FELINE IMMUNODEFICIENCY VIRUS AND THEIR ANTIGENIC RELATIONSHIP WITH EQUINE INFECTIOUS-ANEMIA VIRUS PROTEINS

被引:48
作者
EGBERINK, HF
EDERVEEN, J
MONTELARO, RC
PEDERSEN, NC
HORZINEK, MC
KOOLEN, MJM
机构
[1] STATE UNIV UTRECHT,FAC VET,INST INFECT DIS & IMMUNOL,DEPT VIROL,YALELAAN 1,3584 CL UTRECHT,NETHERLANDS
[2] UNIV CALIF DAVIS,SCH VET MED,DAVIS,CA 95616
[3] LOUISIANA STATE UNIV,DEPT BIOCHEM,BATON ROUGE,LA 70803
关键词
D O I
10.1099/0022-1317-71-3-739
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Feline immunodeficiency virus (FIV) grown in cat lymphocyte and thymocyte cultures was labelled with L-[35S]methionine or [3H]glucosamine and virus-coded proteins were identified using immunoprecipitation. Polypeptides with apparent M(r) values of 15K, 24K, 43K, 50K, 120K and 160K were detected. An additional polypeptide of 10K was detected by Western blot analysis. The two highest M(r) species sometimes appeared as one band, of which only the 120K polypeptide was glycosylated. In the presence of tunicamycin gp 120 was no longer detectable and a non-glycosylated precursor of 75K was found instead. Pulse-chase experiments suggested that the smaller polypeptides p24 and p15 are cleavage products of both p160 and p50. Western blot analysis using a rabbit serum directed against p26 of equine infectious anaemia virus (EIAV) and an anti-EIAV horse serum from a field case of infection revealed a cross-reactivity with p24 of FIV. Cat sera collected late after experimental FIV infection recognized p26 of EIAV, indicating a reciprocal cross-reactivity.
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收藏
页码:739 / 743
页数:5
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