DIURNAL RHYTHM IN SERUM ACTIVITY OF WHEAT-GERM LECTIN-PRECIPITABLE ALKALINE-PHOSPHATASE - TEMPORAL RELATIONSHIPS WITH THE DIURNAL RHYTHM OF SERUM OSTEOCALCIN

被引：23

作者：

NIELSEN, HK

BRIXEN, K

MOSEKILDE, L

机构：

[1] University Department of Endocrinology and Metabolism, Aarhus Amtssygehus

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1990年 / 50卷 / 08期

关键词：

ALKALINE PHOSPHATASE; BONE ISOENZYME; BONE MARKERS; CIRCADIAN RHYTHM; OSTEOBLASTIC ACTIVITY;

D O I：

10.3109/00365519009104952

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Serum levels of osteocalcin (S-OC) and lectin-precipitable alkaline phosphatase activity (S-LAP) are sensitive markers of osteoblastic activity. Diurnal variation has been found for S-OC but has not been reported for S-LAP. We measured S-LAP and serum total alkaline phosphatase (S-TAP) in samples drawn every 60 min during a 24-h study period in nine normal subjects and correlated the findings with the diurnal variation in S-OC. A significant (p < 0.05) diurnal variation in S-LAP characterized by peaks at 1430 hours and 2330 hours and nadir at 0630 hours was found. Peak levels were 30% higher than nadir level (p < 0.05). S-TAP also varied significantly (p < 0.05) with nadir at 0630 hours, showing a difference of 23% between peak and nadir levels (p < 0.05). Significant cross-correlation was found between S-OC and S-LAP and S-TAP when these lagged 4 h after S-OC: r = 0.51 (p < 0.02) and r = 0.65 (p < 0.003), respectively. In other words, changes in S-LAP and S-TAP preceded changes in S-OC by 4 h. There were no significant cross-correlations between the non-lectin-precipitable fraction of AP and S-OC. In conclusion, S-LAP varies in a diurnal rhytm closely related with the diurnal rhythm of S-OC. The almost similar patterns in the diurnal serum levels of these two osteoblastic products strongly suggest that osteoblastic activity fluctuates rhythmically during the day in humans.

引用

页码：851 / 856

页数：6

共 30 条

[1]

Simmons D.J., Whiteside L.A., Whitson S.W., Bio-rhythmic profiles in the rat skeleton, Metab Bone Dis Rel Res, 2, pp. 49-64, (1979)

[2]

Hansson L.I., Stenstrom A., Thorngren K.G., Diurnal variation of longitudinal bone growth in the rabbit, Acta Orthop Scand, 45, pp. 499-507, (1974)

[3]

Simmons D.J., Daily rhythm of 35S incorporation into epiphyseal cartilage in mice, Experientia, 24, (1968)

[4]

Poser J.W., Esch F.S., Ling N.C., Price P.A., Isolation and sequence of the vitamin K-dependent protein from human bone, J Biol Chem, 255, pp. 8685-8691, (1980)

[5]

Price P.A., Williamson M.K., Lothringer J.W., Origin of the vitamin K-dependent bone protein found in plasma and its clearance by kidney and bone, J Biol Chem, 256, pp. 12760-12766, (1981)

[6]

Markowitz M.E., Gundberg C.M., Rosen J.F., The circadian rhythm of serum osteocalcin concentrations: Effects of 1,25 dihydroxyvitamin D administration, Calcif Tissue Int, 40, pp. 179-183, (1987)

[7]

Nielsen H.K., Charles P., Mosekilde L., The effect of single oral doses of prednisone on the circadian rhythm of serum osteocalcin in normal subjects, J Clin Endocrinol Metab, 67, pp. 1025-1030, (1988)

[8]

Charles P., Poser J.W., Mosekilde L., Jensen F.T., Estimation of bone turnover evaluated by <sup>47</sup>Ca-kinetics. Efficiency of serum bone gamma-carboxyglutamic acid-containing protein, serum alkaline phosphatase, and urinary hydroxyproline excretion, J Clin Invest, 76, pp. 2254-2258, (1985)

[9]

Delmas P.D., Malaval L., Arlot M.E., Meunier P.J., Serum bone gla-protein compared to bone histo-morphometry in endocrine diseases, Bone, 6, pp. 339-341, (1985)

[10]

Garcia-Carrasco M., Gruson M., De Vernejoul C., Denne A., Miravet L., Osteocalcin and bone histomorpholometric parameters in adults without bone disease, Calcif Tissue Int, 42, pp. 13-17, (1988)

← 1 2 3 →