PURIFICATION OF AN ENDOGENOUS MODULATOR FOR VOLTAGE-SENSITIVE CALCIUM CHANNELS USING THE ECCENTRIC MULTILAYER COIL PLANET CENTRIFUGE

被引：1

作者：

HANBAUER, I ^{[1
]}

WRIGHT, AG ^{[1
]}

ITO, Y ^{[1
]}

机构：

[1] NHLBI, CHEM LAB, BETHESDA, MD 20892 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1990年 / 13卷 / 12期

关键词：

D O I：

10.1080/01483919008049038

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-speed countercurrent chromatography using a two-phase solvent system was found to be the method of choice for purifying an endogenous Ca2+ channel modulator. The solvent system composed of n-butanol, acetic acid and water (4:1:5) was most suitable for resolving the active material from other unknown substances of low molecular weight. Countercurrent chromatography proved useful in validating the presence in rat brain of an endogenous ligand for dihydropyridine-sensitive Ca2+ channels. Moreover, the degree of purity of the active material obtained by countercurrent chromatography is suitable for consecutive analysis by mass spectrometry or nuclear magnetic resonance. © 1990, Taylor & Francis Group, LLC. All rights reserved.

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页码：2363 / 2372

页数：10

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