AGE-DEPENDENT MODIFICATION OF LIPID-COMPOSITION AND LIPID STRUCTURAL ORDER-PARAMETER OF RAT PERITONEAL MACROPHAGE MEMBRANES

The effect of aging on the lipid composition and fluidity of rat peritoneal macrophage membranes has been determined using young (3 months), mature (12 months) and aged (24 months) Wistar rats. In the aged animals, total phospholipid decreased significantly (P < 0.05), whereas cholesterol increased (P < 0.01), with an age-dependent increase in the molar ratio of cholesterol/phospholipid. The most marked change in phospholipid content was the significant (P < 0. 001) age-dependent increase of phosphatidylserine and cardiolipin and the significant decrease of phosphatidylcholine and phosphatidylinositol. During aging there was a considerable decrease in arachidonic acid and docosapentanoic acid (about 50% in both cases). In contrast, an increase in the levels of oleic, linolenic and docosahexanoic acid was observed. Steady-state fluorescence polarization using 1,6-diphenyl-1,3,5-hexatriene as the probe was used to estimate the lipid structural order parameter of macrophage membranes. There was a highly significant (P < 0.001) age-dependent increase in the lipid structural order parameter, which correlated well with the increased molar ratio of cholesterol/phospholipid in the membranes isolated from aged animals. The data suggests alteration in membrane lipid-protein interactions in aging, and are consistent with the hypothesis of the aging process.