CLONING OF CDNAS FOR CECROPIN-A AND CECROPIN-B, AND EXPRESSION OF THE GENES IN THE SILKWORM, BOMBYX-MORI

被引：41

作者：

YAMANO, Y ^{[1
]}

MATSUMOTO, M ^{[1
]}

INOUE, K ^{[1
]}

KAWABATA, T ^{[1
]}

MORISHIMA, I ^{[1
]}

机构：

[1] TOTTORI UNIV, FAC AGR, METAB BIOCHEM LAB, TOTTORI 680, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 08期

关键词：

D O I：

10.1271/bbb.58.1476

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones coding cecropins A and B were isolated from a cDNA library constructed from the fat body of immunized Bombyx mori larvae. The cloned cDNAs had an open reading frame of 63 amino acids, indicating the primary translated peptides were processed to form mature cecropins of 35 amino acid residues. The homology in the coding regions of cecropins A and B was 73%. In immunized fat body, the expression of both cecropin A and B genes reached the maximal level 5h after the injection of soluble peptidoglycan, and the high level was maintained until 9 h after immunization. The cecropin A and B genes were expressed at high levels in fat body and hemocytes, at lower but significant levels in malpighian tube, slightly in midgut, and none in silk gland.

引用

页码：1476 / 1478

页数：3

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