INTRAPHAGOCYTIC GROWTH INDUCES AN ANTIBIOTIC-RESISTANT PHENOTYPE OF LEGIONELLA-PNEUMOPHILA

The antimicrobial susceptibilities of Legionella pneumophila isolates grown either in U937 human monocytic cells or in Acanthamoeba polyphaga were studied after release from the host cells without further subculture, Time-survival studies showed that exposure of L. pneumophila cells, grown exclusively in vitro, to 5 mu g of rifampin per ml resulted in at least 99.9% killing after 6 h and no detectable survivors at 24 h. Similar rates of killing were observed for in vitro-grown cells tested by exposure to ciprofloxacin, Conversely, time-survival studies revealed that macrophage-grown and amoeba-grown cells were ca, 1,000-fold more resistant to the activities of both drugs, Macrophage-grown cells treated with 5 mu g of rifampin per mi showed 70 and 62% survival after 6 and 24 h, respectively, Intracellularly grown legionellae were also highly resistant to erythromycin (8 mu g/ml). After 24 h of exposure to the drug, there was 70 and 60% survival for amoeba-grown and macrophage-grown legionellae, respectively, whereas in vitro-grown cells showed a 2-log(10) reduction in viable count, When intracellularly grown L. pneumophila cells were subcultured in broth for 48 h, they reverted to the phenotype characteristic of in vitro growth, Morphologically, the cells were larger than their intracellularly grown counterparts and resistance characteristics were lost, The susceptibilities of the subcultured cells to all three drugs were similar to those of Legionella cells grown exclusively in vitro, In view of these findings, the successful treatment of Legionnaires' disease may be related as much to the resistance phenotype induced by intramacrophage growth as to the ability of the antibiotic to enter phagocytic cells.