ISOLATION AND CHARACTERIZATION OF A LOCUS FROM AZOSPIRILLUM-BRASILENSE SP7 THAT COMPLEMENTS THE TUMORIGENIC DEFECT OF AGROBACTERIUM-TUMEFACIENS CHVB MUTANT

被引：6

作者：

RAINA, S ^{[1
]}

RAINA, R ^{[1
]}

VENKATESH, TV ^{[1
]}

DAS, HK ^{[1
]}

机构：

[1] JAWAHARLAL NEHRU UNIV,CTR BIOTECHNOL,GENET ENGN UNIT,NEW DELHI 110067,INDIA

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1995年 / 8卷 / 02期

关键词：

DNA BASE SEQUENCE; LACZ FUSION; RHIZOBIUM MELILOTI NDVB LOCUS;

D O I：

10.1094/MPMI-8-0322

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromosomal virulence gene chvB of Agrobacterium tumefaciens is required for pathogenesis. A DNA fragment from the chvB locus can hybridize to DNA from Azospirillum hm brasilense Sp7. This DNA fragment could restore the tumorigenic activity of the chvB mutant strain A. tumefaciens A1011 towards leaf disks of Nicotiana tabacum. An NH2-terminal open reading frame, 480 codons long, was most likely responsible for the restoration of the tumorigenic activity. The A. brasilense sequence showed good homology with the NH2-terminal region of the ndvB gene of Rhizobium meliloti.

引用

页码：322 / 326

页数：5

共 14 条

[1] EXPRESSION OF THE AGROBACTERIUM-TUMEFACIENS CHVB VIRULENCE REGION IN AZOSPIRILLUM SPP [J].

ALTABE, S ;

DEIANNINO, NI ;

DEMENDOZA, D ;

UGALDE, RA .

JOURNAL OF BACTERIOLOGY, 1990, 172 (05) :2563-2567

[2]

DITTA G, 1995, PLASMID, V13, P144

[3] IDENTIFICATION AND GENETIC-ANALYSIS OF AN AGROBACTERIUM-TUMEFACIENS CHROMOSOMAL VIRULENCE REGION [J].

DOUGLAS, CJ ;

STANELONI, RJ ;

RUBIN, RA ;

NESTER, EW .

JOURNAL OF BACTERIOLOGY, 1985, 161 (03) :850-860

[4] RHIZOBIUM-MELILOTI GENES REQUIRED FOR NODULE DEVELOPMENT ARE RELATED TO CHROMOSOMAL VIRULENCE GENES IN AGROBACTERIUM-TUMEFACIENS [J].

DYLAN, T ;

IELPI, L ;

STANFIELD, S ;

KASHYAP, L ;

DOUGLAS, C ;

YANOFSKY, M ;

NESTER, E ;

HELINSKI, DR ;

DITTA, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4403-4407

[5] MOLECULAR-BIOLOGY AND ECOLOGY OF DIAZOTROPHS ASSOCIATED WITH NON-LEGUMINOUS PLANTS [J].

ELMERICH, C .

BIO-TECHNOLOGY, 1984, 2 (11) :967-978

[6]

EYERS M, 1988, FEMS MICROBIOL LETT, V49, P435, DOI 10.1111/j.1574-6968.1988.tb02771.x

[7]

IELPI L, 1990, J BIOL CHEM, V265, P2843

[8] CHANGES IN ROOT MORPHOLOGY OF WHEAT CAUSED BY AZOSPIRILLUM INOCULATION [J].

KAPULNIK, Y ;

OKON, Y ;

HENIS, Y .

CANADIAN JOURNAL OF MICROBIOLOGY, 1985, 31 (10) :881-887

[9] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].

KYTE, J ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132

[10]

Miller J.H., 1972, EXPT MOL GENETICS, P1

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