THE EFFECT OF FK506 ON WARM ISCHEMIA AND REPERFUSION INJURY IN THE RAT-LIVER

被引：21

作者：

WAKABAYASHI, H

KARASAWA, Y

TANAKA, S

KOKUDO, Y

MAEBA, T

机构：

[1] First Department of Surgery, Kagawa Medical School, Miki-cho, Kita-gun, Kagawa

来源：

SURGERY TODAY-THE JAPANESE JOURNAL OF SURGERY | 1994年 / 24卷 / 11期

关键词：

LIVER; ISCHEMIA AND REPERFUSION INJURY; FK506; 31P-MRS; ICAM-1;

D O I：

10.1007/BF02215813

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

The protective effect of FK506 on hepatocytes against ischemia and reperfusion injury was examined by evaluating the following: the high energy phosphorus metabolism obtained using P-31 magnetic resonance spectroscopy (P-31-MRS) and the tissue blood flow of the liver in ischemia and the reperfusion process, mitochondrial glutamic oxaloacetic transaminase (m-GOT) and glutamic pyruvic transaminase (GPT), the survival rates of the animals, a histological study and immunohistological staining for intercellular adhesion molecule-1 (ICAM-1) in the liver after ischemia. The rats were treated with FK506 1mg/kg/day i.m. for 4 days before testing. Ischemia was induced by clamping the hepatoduodenal ligament for 30 min. In P-31-MRS, the recovery of the hepatic energy status after ischemia, evaluated by beta-ATP/inorganic phosphate (Pi), was significantly better in the FK506 group. It also coincided with the recovery of tissue blood Bow monitored with a laser Doppler flowmeter. In the histological examination, the congestion observed in the periportal region of the control group was mild, while there was less induction of ICAM-1 in the endothelial cells of the portal veins and hepatic veins in the FK506 group. From these findings, we concluded that FK506 had a protective effect on hepatocytes against warm ischemia and reperfusion injury, and the mechanism for this could partially be attributed to improved tissue blood Bow after ischemia by the modulation of immunological events.

引用

页码：994 / 1002

页数：9

共 25 条

[1]

Wakabayashi H., Karasawa Y., Maeba T., Tanaka S., Effect of FK506 and cyclosporine A on hepatic energy status in the rat after warm ischemia, as monitored by<sup>31</sup>P nuclear magnetic résonance spectroscopy in vivo, Transplant Proc, 24, pp. 1993-1995, (1992)

[2]

Dhar D.K., Nagasue N., Kimoto T., Uchida M., Takemoto Y., Nakamura T., The salutary effect of FK506 in ischemiareperfusion injury of the canine liver, Transplantation, 54, pp. 583-588, (1992)

[3]

Francavilla A., Barone M., Todo S., Zeng Q., Porter K.A., Starzl T.E., Augmentation of rat liver regeneration by FK506 compared with cyclosporine, Lancet, 25, pp. 1248-1249, (1989)

[4]

Starzl T.E., Porter K.A., Mazzaferro V., Todo S., Fung J., Francavilla A., Hepatotropic effects of FK506 in dogs, Transplantation, 51, pp. 67-70, (1991)

[5]

Mazzaferro V., Scotti-Foglieni C.L., Porter K.A., Trejo-Bellido J., Carrieri G., Todo S., Fung J.J., Francavilla A., Starzl T.E., Studies of hepatotropic qualities of FK506 and CyA, Transplant Proc, 22, pp. 93-95, (1990)

[6]

Sakr M.F., Zetti G.M., Farghali H., Hassanein T.H., Gavaler J.S., Starzl T.E., Van Thiel D.H., Protective effect of FK506 against hepatic ischemia in rats, Transplant Proc, 23, pp. 340-341, (1991)

[7]

Sakr M.F., Zetti G.M., Hassanein T.I., Farghali H., Nalesnik M.A., Gavaler J.S., Starzl T.E., Van Thiel D.H., FK506 ameliorates the hepatic injury associated with ischemia and reperfusion in rats, Hepatology, 13, pp. 947-951, (1991)

[8]

Starzl T.E., Todo S., Fung J., Demetris A.J., Venkataramman R., Jain A., FK506 for liver, kidney, and pancreas transplantation, Lancet, 2, pp. 1000-1004, (1989)

[9]

Bore P.J., Paratheofanis I., Sells R.A., Adenosine triphosphate regeneration and function in the rat kidney following warm ischemia, Transplantatoin, 27, pp. 235-237, (1979)

[10]

Marubayashi S., Takenaka M., Dohi K., Ezaki H., Kawasaki T., Adenine nucleotide metabolism during hepatic ischemia and subsequent blood reflow periods and its relation to organ viability, Transplantation, 30, pp. 294-296, (1980)

← 1 2 3 →