PREPARATION OF POLYDEOXYNUCLEOTIDES LINKED TO A SOLID SUPPORT BY COUPLING CNBR-ACTIVATED CELLULOSE WITH 5'-NH2-TERMINATED OLIGO AND POLY(PDTS)

被引：7

作者：

CLERICI, L ^{[1
]}

CAMPAGNARI, F ^{[1
]}

ROOIJ, JFMD ^{[1
]}

VANBOOM, JH ^{[1
]}

机构：

[1] GORLAEUS LAB,NL-2300 RA LEIDEN,NETHERLANDS

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 01期

关键词：

D O I：

10.1093/nar/6.1.247

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Synthetically-prepared 5′-NH2-dT(pdT)noligomers (6b,n=4 or 7) were immobilized on cyanogen bromide activated cellulose. The influence of temperature, pH, and ionic strength on the rate of the coupling process was studied. The oligomer 5′-NH2-dT(pdT)8could be elongated enzymatically to the polymers 5′-NH 2.-dT(pdT)n(n=20, 51 and 84), which could be immobilized on cellulose. The cellulose-NH-dT(pdT)84, polymer thus obtained could be assembled to a new solid-state polymer e.g. poly(dA)290poly|3H|dT)200, poly(dT)85-cellulose which, in turn, was a very convenient substrate for assaying DNA-ligase. © 1979 Information Retrieval Limited.

引用

页码：247 / 258

页数：12

共 26 条

[1] COVALENT ATTACHMENT OF DNA TO AGAROSE - IMPROVED SYNTHESIS AND USE IN AFFINITY CHROMATOGRAPHY [J].