DETECTION OF LIPID-PEROXIDATION IN LUNG AND IN BRONCHOALVEOLAR LAVAGE CELLS AND FLUID

被引：32

作者：

PETRUSKA, JM

WONG, SHY

SUNDERMAN, FW

MOSSMAN, BT

机构：

[1] Department of Pathology, University of Vermont College of Medicine, Burlington

[2] Department of Laboratory Medicine, University of Connecticut Scool of Medicine, Farmington

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 9卷 / 01期

关键词：

Aldehydes; Bronchoalveolar lavage; Chromatography (High pressure liquid); Free radicals; Lipid peroxidation; Lung;

D O I：

10.1016/0891-5849(90)90049-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhalation of toxic materials such as asbestos, silica, 100% oxygen, ozone, or nitrogen dioxide may lead to an increased production of reactive oxygen metabolites which may initiate lipid peroxidation. Measurement of lipid peroxidation in cells and fluid obtained by bronchoalveolar lavage (BAL), as well as lung tissue, may aid in monitoring the development and extent of pulmonary damage after inhalation of a toxic substance. In this study, we employed a sensitive assay for detection of malondialdehyde (MDA), a breakdown product of lipid peroxidation. By separation of the adduct with thiobarbituric acid, using a reverse phase high pressure liquid chromatographic technique, we accurately and sensitively measured the content of MDA in BAL cells, lavage fluid, and lavaged lung tissue homogenates of rats. The amounts of sample required for detection of MDA were small enough possibly to be applied to use with human specimens; in adiition, recovery of added MDA was acceptable with all types of samples. Inclusion of a metal chelator in the preparation of samples appeared necessary to prevent metal-catalyzed propagation of lipid peroxidation during the assay. Overall, the method described here using samples from rats may be applicable to detecting lipid peroxidation in BAL samples from humans. © 1990.

引用

页码：51 / 58

页数：8

共 31 条

[1]

Beuge, Aust, Microsomal lipid peroxidation, Methods Enzymol., 52, pp. 302-310, (1978)

[2]

Marshall, Warso, Lands, Selective microdetermination of lipid hydroperoxides, Anal. Biochem., 145, pp. 192-199, (1985)

[3]

Warso, Lands, Presence of lipid hydroperoxides in human plasma, J. Clin. Invest., 75, pp. 667-671, (1985)

[4]

Yamamoto, Frei, Ames, Assay of lipid hydroperoxides using HPLC with a post-column chemiluminescence detection system, Methods Enzymol., (1990)

[5]

Girotti, Mechanisms of lipid peroxidation, J. Free Radic. Biol. Med., 1, pp. 87-95, (1985)

[6]

Hiatt, Hydroperoxide destroyers and how they work, C R C Critical Reviews in Food Science and Nutrition, 7, pp. 1-12, (1975)

[7]

Recknagel, Glende, Spectrophotometric detection of lipid conjugated dienes, Methods Enzymol., 105, pp. 331-337, (1984)

[8]

Knight, Hopfer, Reid, Wong, Sunderman, Ethene (ethylene) and ethane exhalation in Ni[II]-treated rats, using an improved rebreathing apparatus, Ann. Clin. Lab. Med., 16, pp. 386-394, (1986)

[9]

Malshet, Tappel, Fluorescent products of lipid peroxidation: I. structural requirements for fluoresnce in conjugated Schiff bases, Lipids, 8, pp. 194-198, (1973)

[10]

Reiss, Tappel, Fluorescent product formation and changes in structure of DNA reacted with peroxiding arachidonic acid, Lipids, 8, pp. 199-202, (1973)

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