CLONING OF MYCOBACTERIAL HISTIDINE SYNTHESIS GENES BY COMPLEMENTATION OF A MYCOBACTERIUM-SMEGMATIS AUXOTROPH

被引：22

作者：

HINSHELWOOD, S ^{[1
]}

STOKER, NG ^{[1
]}

机构：

[1] UNIV LONDON LONDON SCH HYG & TROP MED,DEPT CLIN SCI,BACTERIAL MOLEC GENET UNIT,KEPPEL ST,LONDON WC1E 7HT,ENGLAND

来源：

MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 19期

基金：

英国惠康基金;

关键词：

D O I：

10.1111/j.1365-2958.1992.tb01468.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Histidine-requiring auxotrophs of Mycobacterium smegmatis were isolated following N-methyl-N'-nitro-N-nitrosoguanidine treatment. One of these mutants, his5, was transformed with an M. smegmatis shuttle cosmid library, and complementing clones were isolated at a frequency of approximately 1%. A 2.3 kb fragment was subcloned and sequenced, and found to contain the start of an operon including the hisD gene and part of the hisC gene. No hisG gene was detected upstream of hisD, suggesting that the regulation of histidine biosynthesis in mycobacteria may differ from that of Escherichia coli. The strategy used here will allow the molecular genetics of complex mycobacterial-specific biosynthetic pathways involved in the virulence of pathogenic species to be studied.

引用

页码：2887 / 2895

页数：9

共 41 条

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