CLONING AND SEQUENCING OF THE URA5 GENE FROM THE YEAST YARROWIA-LIPOLYTICA

被引：14

作者：

SANCHEZ, M

PRADO, M

IGLESIAS, FJ

DOMINGUEZ, A

机构：

[1] UNIV SALAMANCA, DEPT GENET & MICROBIOL, E-37071 SALAMANCA, SPAIN

[2] UNIV SALAMANCA, DEPT FIS APLICADA, E-37071 SALAMANCA, SPAIN

来源：

YEAST | 1995年 / 11卷 / 05期

关键词：

YARROWIA LIPOLYTICA; OROTATE PHOSPHORIBOSYL TRANSFERASE; NUCLEOTIDE SEQUENCE; TRANSCRIPTION;

D O I：

10.1002/yea.320110505

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The URAS gene of Yarrowia lipolytica encoding the orotate phosphoribosyl transferase (OPRTase, EC2.4.2.10) was isolated by target integration in a mutant strain originally named ura2.21. The nucleotide sequence of the gene predicts a protein with high similarities with the OPRTases from Saccharomyces cerevisiae, Podospora anserina and Escherichia coli and to a lesser extent with that of Dictyostelium discoideum. The transcription start point has been mapped by primer extension analysis and indicates the existence of a long leader sequence in the corresponding mRNA. Northern-blot hybridization revealed the URAS transcript to be approximately 0.94 kb. Deletion of the URA5 gene in Y. lipolytica produced a leaky phenotype similar to the one described for the ura5 mutation in S. cerevisiae. The URA5 gene of Y. lipolytica was able to complement functionally the ura5 mutation of S. cerevisiae. The sequence presented here has been submitted to the EMBL data library under Accession Number Z22571.

引用

页码：425 / 433

页数：9

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