ZETA-COP, A SUBUNIT OF COATOMER, IS REQUIRED FOR COP-COATED VESICLE ASSEMBLY

被引：68

作者：

KUGE, O

HARAKUGE, S

ORCI, L

RAVAZZOLA, M

AMHERDT, M

TANIGAWA, G

WIELAND, FT

ROTHMAN, JE

机构：

[1] MEM SLOAN KETTERING CANC CTR, CELLULAR BIOCHEM & BIOPHYS PROGRAM, 1275 YORK AVE, NEW YORK, NY 10021 USA

[2] UNIV GENEVA, SCH MED, INST HISTOL & EMBRYOL, DEPT MORPHOL, CH-1211 GENEVA 4, SWITZERLAND

[3] UNIV HEIDELBERG, INST BIOCHEM 1, W-6900 HEIDELBERG, GERMANY

来源：

JOURNAL OF CELL BIOLOGY | 1993年 / 123卷 / 06期

关键词：

D O I：

10.1083/jcb.123.6.1727

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta', gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.

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页码：1727 / 1734

页数：8

相关论文

共 31 条

[1] A MICROTUBULE-BINDING PROTEIN ASSOCIATED WITH MEMBRANES OF THE GOLGI-APPARATUS [J].

ALLAN, VJ ;

KREIS, TE .

JOURNAL OF CELL BIOLOGY, 1986, 103 (06) :2229-2239

[2] SPECIMEN PREPARATION FOR ELECTRON-MICROSCOPY USING LOW-TEMPERATURE EMBEDDING RESINS [J].

ARMBRUSTER, BL ;

CARLEMALM, E ;

CHIOVETTI, R ;

GARAVITO, RM ;

HOBOT, JA ;

KELLENBERGER, E ;

VILLIGER, W .

JOURNAL OF MICROSCOPY-OXFORD, 1982, 126 (APR) :77-85

[3] RECONSTITUTION OF THE TRANSPORT OF PROTEIN BETWEEN SUCCESSIVE COMPARTMENTS OF THE GOLGI MEASURED BY THE COUPLED INCORPORATION OF N-ACETYLGLUCOSAMINE [J].

BALCH, WE ;

DUNPHY, WG ;

BRAELL, WA ;

ROTHMAN, JE .

CELL, 1984, 39 (02) :405-416

[4] ADP-RIBOSYLATION FACTOR, A SMALL GTP-BINDING PROTEIN, IS REQUIRED FOR BINDING OF THE COATOMER PROTEIN BETA-COP TO GOLGI MEMBRANES [J].

DONALDSON, JG ;

CASSEL, D ;

KAHN, RA ;

KLAUSNER, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6408-6412

[5] BINDING OF ARF AND BETA-COP TO GOLGI MEMBRANES - POSSIBLE REGULATION BY A TRIMERIC G-PROTEIN [J].

DONALDSON, JG ;

KAHN, RA ;

LIPPINCOTTSCHWARTZ, J ;

KLAUSNER, RD .

SCIENCE, 1991, 254 (5035) :1197-1199

[6] BETA-COP, A 110 KD PROTEIN ASSOCIATED WITH NON-CLATHRIN-COATED VESICLES AND THE GOLGI-COMPLEX, SHOWS HOMOLOGY TO BETA-ADAPTIN [J].

DUDEN, R ;

GRIFFITHS, G ;

FRANK, R ;

ARGOS, P ;

KREIS, TE .

CELL, 1991, 64 (03) :649-665

[7] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[8]

HARLOW E, 1988, ANTIBODIES LABORATOR

[9] SEC21 IS A GENE REQUIRED FOR ER TO GOLGI PROTEIN-TRANSPORT THAT ENCODES A SUBUNIT OF A YEAST COATOMER [J].

HOSOBUCHI, M ;

KREIS, T ;

SCHEKMAN, R .

NATURE, 1992, 360 (6404) :603-605

[10] CLATHRIN AND ASSOCIATED ASSEMBLY AND DISASSEMBLY PROTEINS [J].

KEEN, JH .

ANNUAL REVIEW OF BIOCHEMISTRY, 1990, 59 :415-438

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