SUCCESSFUL IN-VITRO FERTILIZATION AND PREGNANCY BY MICROMANIPULATION WITH EPIDIDYMAL SPERM

被引：4

作者：

FUKUGAKI, H ^{[1
]}

SUGANUMA, N ^{[1
]}

KITAGAWA, T ^{[1
]}

YAMAMOTO, M ^{[1
]}

HIBI, H ^{[1
]}

MIYAKE, K ^{[1
]}

TOMODA, Y ^{[1
]}

机构：

[1] NAGOYA UNIV,BRANCH HOSP,SCH MED,DEPT OBSTET & GYNECOL,HIGASHI KU,NAGOYA 461,AICHI,JAPAN

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1994年 / 11卷 / 09期

关键词：

EPIDIDYMAL SPERM; IN VITRO FERTILIZATION AND EMBRYO TRANSFER; MICROMANIPULATION; PARTIAL ZONA DISSECTION; SUBZONAL INSEMINATION;

D O I：

10.1007/BF02215707

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: To examine the effectiveness of micromanipulation on the treatment of the patients with obstructive azoospermia, subzonal insemination or partial zona dissection was performed using epididymal sperm of the 23 patients, and the in vitro fertilization and embryo transfer (IVF-ET) outcomes were analyzed. Results: Fertilized oocytes were obtained in II cycles of the 25 treated cycles, and six pregnancies were established by the subsequent ET. Through the analysis of 13 cycles in which both the micromanipulation and usual IVF insemination were performed, the fertilization rate per oocyte with the micromanipulation [25% (28/113)] was significantly higher than that with the usual insemination method[4% (4/102)]. The micromanipulation was most useful for the treatment of the patients with congenital absence of the vas deferens. Moreover, there was no need to aspirate a large volume of the epididymal fluid, but it was essential to prepare more than 10 mature oocytes retrieved. Conclusions: These results indicate that micromanipulation with epididymal sperm is an effective treatment for obstructive azoospermia, and careful preparations of sperm and well-controlled ovarian hyperstimulation are necessary for successful IVF-ET.

引用

页码：452 / 458

页数：7

共 32 条

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