REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF TENUAZONIC ACID AND RELATED TETRAMIC ACIDS

被引：36

作者：

SHEPHARD, GS ^{[1
]}

THIEL, PG ^{[1
]}

SYDENHAM, EW ^{[1
]}

VLEGGAAR, R ^{[1
]}

MARASAS, WFO ^{[1
]}

机构：

[1] UNIV PRETORIA,DEPT CHEM,PRETORIA 0002,SOUTH AFRICA

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 566卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80124-U

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic system for the determination of the fungal toxin, tenuazonic acid, (5S,8S)-3-acetyl-5-sec.-butyltetramic acid, is described. The system utilizes a column packed with deactivated end-capped C18 silica with a high carbon load to overcome the problem of poor chromatographic performance of this beta-diketone on reversed-phase liquid chromatography which previously necessitated the use of anion-exchange, ligand-exchange or ion-pairing methods. The reversed-phase system allows the separation of tenuazonic acid from its (5R,8S)-diastereomer, allo-tenuazonic acid and was applied to the detection of tenuazonic acid in cultures of Alternaria alternata and Phoma sorghina. By means of diode-array ultraviolet detection, (5S)-3-acetyl-5-isopropyltetramic acid was observed in extracts of culture material. This metabolite was purified using the analytical reversed-phase system and was identified by H-1 and C-13 nuclear magnetic resonance spectroscopy.

引用

页码：195 / 205

页数：11

共 14 条

[1] ISOLATION AND CHARACTERIZATION OF TRICHOTHECIN FROM CORN CULTURES OF FUSARIUM-GRAMINEARUM MRC-1125 [J].