AXENIC STORAGE OF SMALL VOLUMES OF TETRAHYMENA CULTURES UNDER LIQUID-NITROGEN - MINIATURIZED PROCEDURE

被引：12

作者：

FLACKS, M ^{[1
]}

机构：

[1] UNIV CALIF SANTA BARBARA, DEPT BIOL SCI, BIOCHEM & MOLEC BIOL SECT, SANTA BARBARA, CA 93106 USA

来源：

CRYOBIOLOGY | 1979年 / 16卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0011-2240(79)90040-3

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A method for freezing and storing samples of T. thermophila in short lengths of Teflon tubing is described. Survival rates of the organism frozen with this technique are sufficient to permit routine storage of many strains in 1/4 of the liquid N2 refrigerator space previously required.

引用

页码：287 / 291

页数：5

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SANFORD, YM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (07) :3355-3358

[2]

DIETZ A, 1975, ROUND TABLE C CRYOGE, P22

[3]

FLACKS M, UNPUBLISHED

[4] CHARACTERIZATION OF SPECIES OF TETRAHYMENA-PYRIFORMIS COMPLEX [J].

NANNEY, DL ;

MCCOY, JW .

TRANSACTIONS OF THE AMERICAN MICROSCOPICAL SOCIETY, 1976, 95 (04) :664-682

[5]

NILSSON JR, 1974, J CELL SCI, V16, P39

[6]

Orias E, 1976, Methods Cell Biol, V13, P247, DOI 10.1016/S0091-679X(08)61806-3

[7]

PICKETT BW, 1976, 6TH P TECHN C ART IN

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