INDUCTION OF UNSCHEDULED DNA-SYNTHESIS IN SUSPENSIONS OF RAT HEPATOCYTES BY AN ENVIRONMENTAL TOXICANT, 3,3'4,4'-TETRACHLOROAZOBENZENE

被引：26

作者：

HSIA, MTS ^{[1
]}

KREAMER, BL ^{[1
]}

机构：

[1] UNIV WISCONSIN,RUSSELL LABS,CTR ENVIRONM TOXICOL,MADISON,WI 53706

来源：

CANCER LETTERS | 1979年 / 6卷 / 4-5期

关键词：

D O I：

10.1016/S0304-3835(79)80035-X

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Unscheduled DNA synthesis was induced by 3,3′4,4′-tetrachloroazobenzene (TCAB)) in freshly isolated suspensions of rat hepatocytes. A dose-dependent response was demonstrated. Hepatocellular DNA was obtained after the chloroform-isoamyl alchohol-phenol extraction of the isolated nuclei. The induction of unscheduled DNA synthesis was measured by the incorporation of [3H]-thymidine in the presence of hydroxyurea as determined by the scintillation counting assay. DNA repair data obtained in this study on benzo[a]pyrene and methyl methanesulfonate are comparable to a previous report using primary cultures of hepatocytes and cesium chloride gradients. Hence, the present method offers promise as a rapid and sensitive screen for chemical carcinogens. © 1979 Elsevier/North-Holland Scientific Publishers Ltd., All rights reserved.

引用

页码：207 / 212

页数：6

共 12 条

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