CONFORMATIONAL STUDIES ON THE N-LINKED CARBOHYDRATE CHAIN OF BROMELAIN

被引：55

作者：

BOUWSTRA, JB ^{[1
]}

SPOELSTRA, EC ^{[1
]}

DEWAARD, P ^{[1
]}

LEEFLANG, BR ^{[1
]}

KAMERLING, JP ^{[1
]}

VLIEGENTHART, JFG ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,DEPT BIOORGAN CHEM,TRANSITORIUM 3,POB 80075,3508 TB UTRECHT,NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 190卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15553.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1H‐ and 13C‐NMR assignments for the carbohydrate part of the glycopeptide α‐d‐Man‐(1→6)‐[ß‐d‐Xyl‐(1→2)]‐ß‐d‐Man‐(1→4)‐ß‐d‐GlcNAc‐(1→4)‐[α‐l‐Fuc‐(1→3)]‐ß‐d‐ GlcNAc‐(1→N)‐Asn∼, derived from the proteolytic enzyme bromelain (EC 3.4.22.4), have been obtained using homo‐ and heteronuclear correlation spectroscopy, two‐dimensional homonuclear Hartmann‐Hahn and nuclear Overhauser enhancement experiments. A conformational model for the carbohydrate chain, deduced from the NMR data and consistent with hard‐sphere exo‐anomeric calculations shows that the rotamer population about the C‐5–C‐6 bond of ß‐Man is restricted to the Pω=180 rotamer, mainly. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：113 / 122

页数：10

共 47 条

[1] THE BETA-1-]2-D-XYLOSE AND ALPHA-1-]3-L-FUCOSE SUBSTITUTED N-LINKED OLIGOSACCHARIDES FROM ERYTHRINA-CRISTAGALLI LECTIN - ISOLATION, CHARACTERIZATION AND COMPARISON WITH OTHER LEGUME LECTINS [J].

ASHFORD, D ;

DWEK, RA ;

WELPLY, JK ;

AMATAYAKUL, S ;

HOMANS, SW ;

LIS, H ;

TAYLOR, GN ;

SHARON, N ;

RADEMACHER, TW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 166 (02) :311-320

[2] 2-DIMENSIONAL SPECTROSCOPY - APPLICATION TO NUCLEAR MAGNETIC-RESONANCE [J].

AUE, WP ;

BARTHOLDI, E ;

ERNST, RR .

JOURNAL OF CHEMICAL PHYSICS, 1976, 64 (05) :2229-2246

[3] MLEV-17-BASED TWO-DIMENSIONAL HOMONUCLEAR MAGNETIZATION TRANSFER SPECTROSCOPY [J].

BAX, A ;

DAVIS, DG .

JOURNAL OF MAGNETIC RESONANCE, 1985, 65 (02) :355-360

[4] BROAD-BAND HOMONUCLEAR DECOUPLING IN HETERONUCLEAR SHIFT CORRELATION NMR-SPECTROSCOPY [J].

BAX, A .

JOURNAL OF MAGNETIC RESONANCE, 1983, 53 (03) :517-520

[5] REINVESTIGATION OF THE CARBOHYDRATE CHAINS OF CALF FETUIN USING C-13-NMR SPECTROSCOPY [J].

BERMAN, E .

CARBOHYDRATE RESEARCH, 1986, 152 :33-46

[6] THE PREFERRED CONFORMATION OF OLIGOSACCHARIDES DERIVED FROM THE COMPLEX-TYPE CARBOHYDRATE PORTIONS OF GLYCOPROTEINS [J].

BOCK, K ;

ARNARP, J ;

LONNGREN, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1982, 129 (01) :171-178

[7] THE PREFERRED CONFORMATION OF OLIGOSACCHARIDES IN SOLUTION INFERRED FROM HIGH-RESOLUTION NMR DATA AND HARD-SPHERE EXO-ANOMERIC CALCULATIONS [J].

BOCK, K .

PURE AND APPLIED CHEMISTRY, 1983, 55 (04) :605-622

[8] H-1-NMR AND C-13-NMR ASSIGNMENTS FOR STRUCTURAL ELEMENTS OF XYLOSE-CONTAINING N-LINKED OLIGOSACCHARIDES, USING 1D-NMR AND 2D-NMR EXPERIMENTS [J].

BOUWSTRA, JB ;

KEREKGYARTO, J ;

KAMERLING, JP ;

VLIEGENTHART, JFG .

CARBOHYDRATE RESEARCH, 1989, 186 (01) :39-49

[9]

BRAUNSCHWEILER L, 1983, J MAGN RESON, V53, P51

[10] CHARACTERIZATION OF 4 LACTOSE MONOPHOSPHATES BY APPLICATION OF P-31-NMR, C-13-NMR, AND H-1-NMR SPECTROSCOPY [J].

BREG, J ;

ROMIJN, D ;

VANHALBEEK, H ;

VLIEGENTHART, JFG ;

VISSER, RA ;

HAASNOOT, CAG .

CARBOHYDRATE RESEARCH, 1988, 174 :23-36

← 1 2 3 4 5 →