CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION DATA OF THE FAB FRAGMENT OF A MONOCLONAL-ANTIBODY AGAINST APAMIN, A BEE VENOM NEUROTOXIN

被引：5

作者：

DEVAUX, C

DEFENDINI, ML

ALZAR, PM

ABERGEL, C

GRANIER, C

FONTECILLACAMPS, JC

机构：

[1] FAC MED NORD,CNRS,CRISTALLOG & CRISTALLISAT MACROMOLEC BIOL LAB,F-13326 MARSEILLE 15,FRANCE

[2] INST PASTEUR,UNITE IMMUNOL STRUCT,F-75724 PARIS 15,FRANCE

来源：

FEBS LETTERS | 1991年 / 286卷 / 1-2期

关键词：

APAMIN; MONOCLONAL ANTIBODY; FAB PURIFICATION; X-RAY CRYSTALLOGRAPHY;

D O I：

10.1016/0014-5793(91)80941-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fab fragments of anti-apamin monoclonal antibodies have been purified to homogeneity and crystallized. The crystals belong to the monoclinic space group P2(1) with cell dimensions a = 99.0 +/- 0.3 angstrom, b = 137.1 +/- 0.4 angstrom, c = 76.0 +/- 0.2 angstrom and beta = 92.9 +/- 0.9-degrees. They most likely contain four molecules in the asymmetric unit (V(m) = 2.39 angstrom3/Da). The possibility of the existence of non-crystallographic symmetry is discussed.

引用

页码：64 / 66

页数：3

共 23 条

[1] 3-DIMENSIONAL STRUCTURE DETERMINATION OF AN ANTI-2-PHENYLOXAZOLONE ANTIBODY - THE ROLE OF SOMATIC MUTATION AND HEAVY LIGHT CHAIN PAIRING IN THE MATURATION OF AN IMMUNE-RESPONSE [J].