PLAQUE-ASSAY WITH PROTEIN-A-COATED ERYTHROCYTES FOR THE EVALUATION OF HUMAN IMMUNOGLOBULIN-SECRETING CELLS INDUCED BY POKEWEED MITOGEN

被引：19

作者：

LANZAVECCHIA, A ^{[1
]}

SACCHI, G ^{[1
]}

MACCARIO, R ^{[1
]}

VITIELLO, A ^{[1
]}

NESPOLI, L ^{[1
]}

UGAZIO, AG ^{[1
]}

机构：

[1] IST SIEROTERAPICO MILANESE S BELFANTI,I-20143 MILAN,ITALY

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 1979年 / 10卷 / 04期

关键词：

D O I：

10.1111/j.1365-3083.1979.tb01354.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Cells secreting IgM, IgG and IgA were evaluated in cultures of human peripheral blood lymphocytes stimulated in vitro with pokeweed mitogen by a haemolytic plaque assay using protein A‐coated erythrocytes in the presence of class‐specifie antisera. Kinetic study revealed that immunoglobulin‐secreting cells appeared after 3 days of culture and peaked between days 5 and 7. IgM‐secreting cells predominated throughout the culture period. This plaque assay is a useful and sensitive in vitro test for the evaluation of polyclonal B‐cell activation in humans and may provide a good approach to the study of disturbances in the synthesis of the various classes of immunoglobulins. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：297 / 302

页数：6

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