ACRIDINE-ORANGE STAINING OF THE MAMMALIAN FIBROBLAST CELL COAT

被引：15

作者：

TIMAR, J

GYAPAY, G

LAPIS, K

机构：

[1] I. Institute of Pathology and Experimental Cancer Research, Semmelweis Medical University Budapest, Budapest, H-1085

来源：

HISTOCHEMISTRY | 1979年 / 64卷 / 02期

关键词：

D O I：

10.1007/BF00490098

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compunds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric-in vitro-measurements and enzymatic digestions, respectively. © 1979 Springer-Verlag.

引用

页码：189 / 193

页数：5

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