GENERAL AND SELECTIVE ISOLATION PROCEDURE FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ANABOLIC-STEROIDS IN TISSUES

被引：33

作者：

LAGANA, A ^{[1
]}

MARINO, A ^{[1
]}

机构：

[1] UNIV ROME LA SAPIENZA,DEPT CHEM,I-00185 ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 588卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)85010-D

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A multi-residue method has been developed for the determination of anabolic steroids in animal tissue. The analytes are extracted from tissue with methanol and the extract is subjected to two solid-phase extractions, one using a non-specific adsorbing material, such as graphitized carbon black (Carbopack B), and the other Amberlite CG-400 I in the OH form. This procedure allowed the neutral anabolics (testosterone, trenbolone and progesterone) to be isolated and separated from the acidic type (phenolic group), such as diethylstilbestrol, oestradiol, zeranol/zearalenone and their respective metabolites. The determination was effected using high-performance liquid chromatography with different detectors (ultraviolet, fluorimetric and electrochemical). Several analytical parameters were studied: chromatographic conditions, recoveries, evaporation step, solvent flow-rate, cartridges reusability, interference of plastic cartridges. For all the anabolics investigated the recoveries were > 83.6%.

引用

页码：89 / 98

页数：10

共 31 条

[1] A METHOD COMBINING SOLVENT AND GEL EXTRACTION FOR ISOLATION AND PRELIMINARY PURIFICATION OF STEROIDS IN TISSUES [J].