ISOLATED GROWTH CONES STIMULATE PROLIFERATION OF CULTURED SCHWANN-CELLS

被引：5

作者：

DENT, EW ^{[1
]}

IDA, JA ^{[1
]}

YOSHINO, JE ^{[1
]}

机构：

[1] COLGATE UNIV,DEPT PSYCHOL,PROGRAM NEUROSCI,HAMILTON,NY 13346

来源：

GLIA | 1992年 / 5卷 / 02期

关键词：

HEPARITINASE; SUBCELLULAR FRACTIONATION;

D O I：

10.1002/glia.440050204

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A growth cone-enriched fraction was prepared from 3-4 day rat cerebra. Examination of the growth cone fraction by electron microscopy revealed numerous structures circular in appearance that contain a number of features common to neuronal growth cones in vivo. The isolated growth cones stimulated a dose-dependent incorporation of [H-3]-thymidine into cultured Schwann cells in a manner similar to that observed with an axolemma-enriched fraction prepared from adult rat brainstem. The mitogenic activities of both the growth cone fraction and axolemma-enriched fraction were decreased 50% and 20%, respectively, by treatment with heparitinase I. The mitogen for Schwann cells present in the isolated growth cones appears to be similar to that found in axolemma-enriched fractions prepared from adult rats.

引用

页码：105 / 111

页数：7

共 27 条

[1]

ARD MD, 1988, J NEUROSCI, V8, P2844

[2] SCHWANN CELL PROLIFERATION IN DEVELOPING MOUSE SCIATIC NERVE - A RADIOAUTOGRAPHIC STUDY [J].

ASBURY, AK .

JOURNAL OF CELL BIOLOGY, 1967, 34 (03) :735-+

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] GROWTH CONE MOTILITY AND GUIDANCE [J].

BRAY, D ;

HOLLENBECK, PJ .

ANNUAL REVIEW OF CELL BIOLOGY, 1988, 4 :43-61

[5] STUDIES ON CULTURED RAT SCHWANN-CELLS .1. ESTABLISHMENT OF PURIFIED POPULATIONS FROM CULTURES OF PERIPHERAL-NERVE [J].

BROCKES, JP ;

FIELDS, KL ;

RAFF, MC .

BRAIN RESEARCH, 1979, 165 (01) :105-118

[6] MITOGENICITY OF BRAIN AXOLEMMA MEMBRANES AND SOLUBLE FACTORS FOR DORSAL-ROOT GANGLION SCHWANN-CELLS [J].

CASSEL, D ;

WOOD, PM ;

BUNGE, RP ;

GLASER, L .

JOURNAL OF CELLULAR BIOCHEMISTRY, 1982, 18 (04) :433-445

[7] EVIDENCE THAT THE AXOLEMMAL MITOGEN FOR CULTURED SCHWANN-CELLS IS A POSITIVELY CHARGED, HEPARAN-SULFATE PROTEOGLYCAN-BOUND, HEPARIN-DISPLACEABLE MOLECULE [J].

DECOSTER, MA ;

DEVRIES, GH .

JOURNAL OF NEUROSCIENCE RESEARCH, 1989, 22 (03) :283-288

[8] AXOLEMMA-ENRICHED FRACTIONS-ISOLATED FROM PNS AND CNS ARE MITOGENIC FOR CULTURED SCHWANN-CELLS [J].

DEVRIES, GH ;

SALZER, JL ;

BUNGE, RP .

DEVELOPMENTAL BRAIN RESEARCH, 1982, 3 (02) :295-299

[9] FURTHER-STUDIES ON THE MITOGENIC RESPONSE OF CULTURED SCHWANN-CELLS TO RAT CNS AXOLEMMA-ENRICHED FRACTIONS [J].

DEVRIES, GH ;

MINIER, LN ;

LEWIS, BL .

DEVELOPMENTAL BRAIN RESEARCH, 1983, 9 (01) :87-93

[10] FRACTIONATION OF ISOLATED RAT CNS MYELINATED AXONS BY SUCROSE DENSITY GRADIENT CENTRIFUGATION IN A ZONAL ROTOR [J].

DEVRIES, GH ;

ANDERSON, MG ;

JOHNSON, D .

JOURNAL OF NEUROCHEMISTRY, 1983, 40 (06) :1709-1717

← 1 2 3 →