ERROR IN THE CALIBRATION OF THE MGATP CHEMICAL-SHIFT LIMIT - EFFECTS ON THE DETERMINATION OF FREE MAGNESIUM BY P-31 NMR-SPECTROSCOPY

被引：52

作者：

MOSHER, TJ

WILLIAMS, GD

DOUMEN, C

LANOUE, KF

SMITH, MB

机构：

[1] PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT BIOL CHEM,HERSHEY,PA 17033

[2] PENN STATE UNIV,MILTON S HERSHEY MED CTR,COLL MED,DEPT CELLULAR & MOLEC PHYSIOL,HERSHEY,PA 17033

来源：

MAGNETIC RESONANCE IN MEDICINE | 1992年 / 24卷 / 01期

关键词：

D O I：

10.1002/mrm.1910240117

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

The measurement of free intracellular magnesium (Mg2+) using the 31P chemical shifts of ATP requires the use of appropriate calibration solutions to determine the chemical‐shift limits ΔATPαβ and ΔMgATPαβ. Solutions containing excess Mg2+ contain significant amounts of Mg2ATP and yield positive errors in the value of ΔMgATPαβ. For physiological applications this may overestimate free intracellular Mg2+ by as much as 300%. This error may be minimized if appropriate mole ratios of Mg2+/ATP are used to calibrate ΔMgATPαβ. © 1992 Academic Press, Inc. Copyright © 1992 Wiley‐Liss, Inc., A Wiley Company

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页码：163 / 169

页数：7

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